Phylogenetic relationships of five pika species (genus Ochotona) based on mitochondrial DNA restriction maps  

作　　者：于宁 郑昌琳 施立明 王文 兰宏 张亚平 

机构地区：[1]Northwest Plateau Institute of Biology, Chinese Academy of Sciences, Xining 810001, China [2]Laboratory of Cellular and Molecular Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China

出　　处：《Science China(Life Sciences)》1996年第2期113-122,共10页中国科学（生命科学英文版）

基　　金：Project supported by the National Natural Science Foundation of China,the Foundation of the Chinese Academy of Sciences for Taxonomy and Fauna,and Laboratory of Cellular and Molecular Evolution,the Chinese Academy of Sciences.

摘　　要：Restriction site mapping of mitochondrial DNA (mtDNA) with 16 restriction endonucleases was used to examine the phylogenetic relationships of Ochotona cansus, O. huangensis, O. thibetana, O. curzoniae and O. erythrotis. A 1-kb length variation between 0. erythrotis of subgenus Pika and other four species of subgenus Ochotona was observed, which may be a useful genetic marker for identifying the two subgenera. The phylogenetic tree constructed using PAUP based on 61 phylogenetically informative sites suggests that O. aythrotis diverged first, followed by O. cansus, while O. atrzoniae and O. huangensis are sister taxa related to O. thibetana. The results indicate that both O. cansus and O. huangensis should be treated as independent species. If the base substitution rate of pikas mtDNA was 2% per million years, then the divergence time of the two subgenera, Pika and Ochotona, is about 8.8 Ma ago of late Miocence, middle Bao-dian of Chinese mammalian age, and the divergence of the four species in subgenus Ochotona would have occurred about 2.5-4.2 Ma ago, Yushean of Chinese mammalian age. This calculation appears to be substantiated by the fossil record.Restriction site mapping of mitochondrial DNA (mtDNA) with 16 restriction endonucleases was used to examine the phylogenetic relationships of Ochotona cansus, O. huangensis, O. thibetana, O. curzoniae and O. erythrotis. A 1-kb length variation between 0. erythrotis of subgenus Pika and other four species of subgenus Ochotona was observed, which may be a useful genetic marker for identifying the two subgenera. The phylogenetic tree constructed using PAUP based on 61 phylogenetically informative sites suggests that O. aythrotis diverged first, followed by O. cansus, while O. atrzoniae and O. huangensis are sister taxa related to O. thibetana. The results indicate that both O. cansus and O. huangensis should be treated as independent species. If the base substitution rate of pikas mtDNA was 2% per million years, then the divergence time of the two subgenera, Pika and Ochotona, is about 8.8 Ma ago of late Miocence, middle Bao-dian of Chinese mammalian age, and the divergence of the four species in

关 键 词：pitas (Ochotona) mitochondrial DNA RESTRICTION maps taxonomy phytogeny. 

分 类 号：Q959[生物学—动物学]