Protein conformation in solution by three-dimensional fluorescence spectrometry  

作　　者：鄢远 许金钩 陈国珍 

机构地区：[1]Department of Chemistry, Nanchang University, Nanchang 330047, China. [2]Department of Chemistry, Xiamen University, Xiamen 361005, China

出　　处：《Science China Chemistry》1996年第5期527-535,共9页中国科学（化学英文版）

基　　金：Project supported by the National Natural Science Foundation of China

摘　　要：The conformations of bovine serum albumin (USA) and egg albumin (EA) in solution and their conformation changes under different conditions were studied by using three-dimensional fluorescence spectrometry (TDFS) such as three-dimensional fluorescence (TDF) spectra and three-dimensional fluorescence polarization (TDFP) spectra with tryptophan residues in protein molecules as an intrinsic fluorescent probe. The results show that the microenvironment of tryptophan residues of protein molecules in various solutions can be directly indicated and TDFS is an effective tool for studying protein conformation in solution. Meantime, some valuable results were obtained.The conformations of bovine serum albumin (USA) and egg albumin (EA) in solution and their conformation changes under different conditions were studied by using three-dimensional fluorescence spectrometry (TDFS) such as three-dimensional fluorescence (TDF) spectra and three-dimensional fluorescence polarization (TDFP) spectra with tryptophan residues in protein molecules as an intrinsic fluorescent probe. The results show that the microenvironment of tryptophan residues of protein molecules in various solutions can be directly indicated and TDFS is an effective tool for studying protein conformation in solution. Meantime, some valuable results were obtained.

关 键 词：three-dimensional fluorescence SPECTROMETRY BOVINE SERUM ALBUMIN EGG ALBUMIN protein conformation. 

分 类 号：O657.3[理学—分析化学]