Study on DNA conformation using three-dimensional fluorescence spectra of ethidium bromide  

作　　者：鄢远 许金钩 陈国珍 林江 

机构地区：[1]Department of Chemistry, Xiamen University, Xiamen 361005, China

出　　处：《Chinese Science Bulletin》1996年第1期30-33,共4页

基　　金：Project supported by the National Natural Science Foundation of China.

摘　　要：Three-dimensional fluorescence (TDF) spectra of ethidium bromide (EB) in various environments, such as different types of micelle and glycerol solution, have recentiy been studied. In this note, the TDF spectra of EB are used to study the conformation change of calf thymus (CT) DNA and fish sperm (FS) DNA in different conditions.The results show that the TDF spectra of EB can not only be used to fingerprint CT DNA and FS DNA, but also effectively explain the interaction between EB and DNA and indicate the conformation change of DNA in different conditions.

关 键 词：DNA CONFORMATION THREE-DIMENSIONAL FLUORESCENCE SPECTROMETRY ETHIDIUM bromide. 

分 类 号：Q52[生物学—生物化学]