四磨汤对大鼠胃窦平滑肌影响及其机制的研究  

Study on Effect and Mechanism of Simo Decoction on Gastric Antral Smooth Muscle in Rats

作　　者：戴迟兵[1] 刘娜[1] 陈文妹[1] 钱伟[1] 侯晓华[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院消化内科,430022

出　　处：《胃肠病学》2011年第10期605-608,共4页Chinese Journal of Gastroenterology

基　　金：国家973计划项目(2009CB523002)资助

摘　　要：背景:临床证实四磨汤可改善胃肠动力障碍疾病的症状,但机制不明。目的:研究四磨汤对大鼠离体胃窦平滑肌收缩活动的影响及其机制。方法:处死Sprague-Dawley大鼠后收集胃窦纵行和环行平滑肌条,检测不同剂量四磨汤(1μl、5μl、25μl、50μl、100μl、150μl和200μl)对胃窦平滑肌收缩的影响,同时观察M受体阻断剂阿托品(10^(-6)mol/L)、M受体激动剂乙酰胆碱(10^(-6)mol/L)对四磨汤诱导的胃窦平滑肌收缩的影响。结果:低-中剂量(1～100μl)四磨汤剂量依赖性地诱导大鼠胃窦纵行肌和环行肌收缩增强,但两者之间的作用无明显差异。阿托品可完全阻断四磨汤对胃窦环行肌、纵行肌的促收缩作用,且对环行肌的阻断效应更明显。以乙酰胆碱预处理后,四磨汤对胃窦纵行肌和环行肌的促收缩作用进一步增强,且对环行肌的作用更明显。结论:四磨汤对大鼠胃窦纵行肌和环行肌具有明显的促收缩作用,该作用主要通过M受体介导。Background： Simo Decoction has been proved having the effect of improving symptoms of gastrointestinal dysmotility in clinical studies, but the mechanism is unclear. Aims： To investigate the effect and mechanism of Simo Decoction on the contractive activity of isolated gastric antral smooth muscle in rats. Methods： Antral longitudinal and circular muscle strips were prepared after sacrificing Sprague-Dawley rats. The effect of different concentrations of Simo Decoction （1 μl, 5 μl, 25 μl, 50 μl, 100 μl, 150 μl, 200 μl） on gastric antral smooth muscle contraction was determined, and the effects of M receptor inhibitor atropine （104 mol/L） and M receptor agonist acetylcholine （104 mol/L） on gastric antral smooth muscle contraction induced by Simo Decoction were assessed. Results： Low to moderate doses （1-100 μl） of Simo Decoction dose-dependently increased the contraction of gastric antral longitudinal and circular muscle, and no significant difference was found between longitudinal and circular muscle. After pre-treatment with atropine, the increased contraction of longitudinal and circular muscle induced by Simo Decoction could be completely blocked, especially the circular muscle; however, if pre-treatment with aeetylcholine, Simo Decoction further increased the contraction, especially the circular muscle. Conclusions： Simo Decoction can dose-dependently promote the contraction of gastric antral longitudinal and circular muscle, and this is mainly mediated by activation of M receptor.

关 键 词：四磨汤 胃肠活动 阿托品 乙酰胆碱 

分 类 号：R285.5[医药卫生—中药学]