海狸鼠卵母细胞体外成熟培养初探  

Study on In Vitro Maturation of Nutria Oocytes

作　　者：孙凤俊[1] 邴印忠[1] 高冰[1] 于兴钟[1] 孟庆刚[2] 谭景和[2] 

机构地区：[1]中国科学院黑龙江农业现代化研究所 [2]东北农业大学生物工程系

出　　处：《黑龙江动物繁殖》1996年第3期9-11,共3页Heilongjiang journal of animal reproduction

摘　　要：实验用ＰＭＳＧ或ＰＭＳＧ＋ＨＣＧ处理或未经激素处理的海狸鼠８只，共获卵巢卵母细胞１３８枚。激素处理对获取卵巢卵母细胞的数量没有影响，而对体外成熟发育至卵丘扩展和半成熟阶段有促进作用。三种不同培养液（Ｗｈｉｔｅｎ＋ＦＣＳ；ＴＣＭ１９９＋ＰＭＳＧ＋ＦＣＳ；ＴＣＭ１９９＋ＨＣＧ＋ＦＣＳ）共培养１２５枚卵母细胞，培养后卵丘扩展率及半成熟率分别为５６．５％，４５．７％，４７．６％和２１．７％，１２．３％，９．５％，以Ｗｈｉｔｅｎ液较高（分别为５６．５％和２１．７％），但只有ＴＣＭ１９９＋ＰＭＳＧ＋ＦＣＳ组有２枚卵母细胞出现第一极体。结果表明海狸鼠卵母细胞与其它啮齿动物的卵母细胞一样，能够在体外培养成熟，完成第一次减数分裂。Oocytes were recovered from the nutrias (Myocaster coypus) treated with PMSG of PMSG+HCG, or the nutrias untreated with hormone.The number of oocytes recovered form ovaries was not different between untreated and hoemone-treated nutrias,but the rates of oocytes with expanded cumulus and semi-matured oocytes after culturing in vitro were significantly in hormone-treated nutrias than untreated nutrias. Oocytes were cultured in three kinds of media (Whitten+FCS; TCM-199+PMSG+FCS; TCM-199+HCG+FCS).The rates of oocytes with expanded cumulus and semi-matured oocytes in the three media were 56.6%,45.7%,47.6% and 21.7%,12.3%,9.5%, respectively.However,there were two oocytes with first polar body only in the medium of TCM-199+PMSG+FCS.The results indicated that nutria oocytes,like other rodent oocytes,can be matured in vitro, complete first meiotic division and discharge first polar body.

关 键 词：海狸鼠 卵母细胞 体外成熟 

分 类 号：S814[农业科学—畜牧学]