小鼠淋巴结基质细胞特性及其在辐射损伤修复中的作用  

CHARACTERIZATION OF LYMPH NODE STROMAL CELLS AND THEIR EFFECTS ON RECOVERY OF RADIATION INJURY IN MICE

作　　者：张红梅[1] 于洪臣[1] 于志强[1] 凌翎 刘及[1] 

机构地区：[1]白求恩医科大学,长春130021

出　　处：《中国实验血液学杂志》1995年第3期324-328,共5页Journal of Experimental Hematology

摘　　要：不论是体外器官培养,还是液体单层细胞培养,基质细胞都是在实质细胞退化死亡基础上不断增殖。单层培养时可观察到多角形及纺锤形细胞散在分布,也可形成集落或单层。器官培养时可形成“滋养细胞”等。在淋巴结基质细胞上可见有造血免疫细胞生长,说明在培养条件下基质细胞可支持实质细胞的生长。0.75Gy照射后,基质细胞(合基质祖细胞)与未照射组对比没有减少,甚至有所增加,而1.5Gy、4.0Gy和8.0Gy照射后其数量显著下降。淋巴结基质祖细胞的D_0值是2.7Gy,n值是1.Characterization and dynamic observation of lymph node stromal cells with either organ culture or liquid monolayer cell culture in vitro were carried out. Diffused polygonal cells or fusiform cells were seen in monolayer cell culture. Stromal cells could also form colonies or single-layers, but in special shapes (i.e. nurse cells) in organ culture. It was observed that the proliferation of hematopoietic immunocytes were over the variety of lymph node stromal cells. It showed that stromal cells could support the growth and proliferation of parenchymal cells. Compared with non-irradiation group, the stromal cell number of irradiation group (0.75Gy) (including stromal precursor cell) did not decrease but slightly increased. When irradiated with 1.5Gy, 4.0Gy and 8.0Gy X-ray the number significantly decreased, the D0 and n values were 2.7Gy and 1.5 respectively, for lymph node stromal precursor cells.

关 键 词：淋巴结基质细胞 造血微环境 细胞培养 辐射敏感性 

分 类 号：R392[医药卫生—免疫学]