小蘖胺免疫抑制作用及其作用机制的探讨  

Study on Immunosuppressive Effects of Berbamine and Its Mechanism

作　　者：罗崇念[1] 林新[1] 曹丽[1] 王立为[1] 赵杨 谢蜀生[2] 肖培根[1] 

机构地区：[1]中国医学科学院药用植物资源开发研究所,北京100094 [2]北京医科大学免疫室

出　　处：《中国中西医结合杂志》1995年第S1期217-219,393,共4页Chinese Journal of Integrated Traditional and Western Medicine

摘　　要：本研究观察了小蘖胺对小鼠免疫功能的抑制作用。结果发现小蘖胺能在体内外抑制小鼠脾细胞对刀豆蛋白 A 和脂多糖的增殖反应,明显减少鼠脾细胞所致的溶血空斑细胞数,减低同种异型抗原诱导的迟发型超敏反应和混合淋巴细胞反应。用流式细胞仪对小鼠脾脏 T 淋巴细胞亚群的分析结果表明:小蘖胺能降低小鼠脾脏 CD_4^+/CD_8^+的比值。说明小蘖胺对小鼠免疫功能的抑制作用可能在于改变 T_H/T_S 细胞的平衡,使抑制活性增强,从而发挥其对免疫应答的负调节作用。The immunosuppressive effects of Berbamine on the murine immune functions were observed.The results showed that Berbamine could significantly inhibit the proliferative responses of murine spleen cells to Con A and Lipopolysaccharide (LPS) reduce the amounts of hemolytic plaque forming cells of murine spleen cells and also decrease the delayed- type hypersensitivity (DTH) and mixed lymphocytes reaction (MLR) induced by allogenic antigen.Furthermore.it showed that Berbamine could decrease the ratio of CD_4^+/SD_8^+ subsets of murine spleen T lymphocytes by FACS assay which might be one of the mechanisms of immunosuppressive effects of Berbamine.

关 键 词：小蘖胺 细胞免疫 体液免疫 机制探讨 

分 类 号：R285.5[医药卫生—中药学]