西红柿CU／ZnSOD基因在大肠杆菌中高效表达的研究  

HIGH LEVEL EXPRESSION OF Cu/Zn SOD OF TOMATO IN ESCHERICHIA COLI

作　　者：王宇光[1] 郭建春[1] 郑学勤[1] 

机构地区：[1]中国热带农业科学院热带作物生物技术国家重点实验室

出　　处：《热带作物学报》1995年第S1期83-88,共6页Chinese Journal of Tropical Crops

摘　　要：利用带有Ｔｒｐ启动子的大肠杆菌表达载体ＷＣＣ８能有效地表达西红柿Ｃｕ／ＺｎＳＯＤ基因，该表达蛋白在菌体中具有活性，并在一定程度上抑制受体菌ＳＯＤ基因的表达。据ＳＤＳ－ＰＡＧＥ分析，表达ＳＯＤ蛋白占茵体总蛋白２０％左右，其活性达３．０×１０５Ｖ／Ｌ发酵液。还利用ＮＢＴ法对表达ＳＯＤ蛋白的活性进行了研究。The CV/Zn SOD(superoxide dismutase)gene of Tomato has been successfully cloned and ex-pressed in E. coli under the transcriptional control of the Trp promoters. The expression protein ofSOD gene prOduced was as much as 20%of total cellular protein by SDS-pAGE eletrophoresisand it showed activity in the host ceIls of E. coli by NBT method,while the expression of SODgene of the host cells was greatly inhibited.It was demonstrated that 3.0 × 105 U activity of SODcould be obtained from 1 LB medium after fermentation for 10h.

关 键 词：CU/ZN SOD基因 大肠杆菌 高效表达 

分 类 号：S641.2[农业科学—蔬菜学]