美人梅的茎段离体培养  

The Stem Segments Culture of Prunus mume cv.Meiren Mei in Vitro

作　　者：傅萼辉[1] 徐惠珠[1] 王豫兰 杨玉英 赵守边 

机构地区：[1]中国科学院武汉植物研究所,430074 [2]武汉市东湖风景区管理局,430074

出　　处：《北京林业大学学报》1995年第S1期75-78,共4页Journal of Beijing Forestry University

摘　　要：取‘美人’梅(Prunus mume cv.Meiren Mei)盆栽母树嫩枝茎段(1～1.5cm)用70%的酒精、0.1%的昇汞水溶液消毒后,接种于WB+KT 2mg/l+NAA 0.05mg/l培养基上置培养室静培养.培养室温度控制在25±3℃,光照为1 000～2 000lx,每日光照12h.培养25天后,茎段上腋芽萌发;再培养25天,这些芽转变成丛生芽.丛生芽在WB+BA 0.5mg/l+NAA 0.025mg/l培养基上培养25天后,它们便长成正常的幼苗.幼苗在WB+BA 0.025mg/l+NAA 0.05mg/l+IAA0.025mg/l培养基上诱导生根.春末夏初时,生根小植株盆栽或温室栽植生长良好,成活率约为80%.已获3 000株苗在全国多点试种,约有半数以上植株已始花.Young stem segments,1～1.5cm in length were surface-sterilized in 70% al- cohol and in 0.1% HgCl_2 water solution and cultured on WB basal medium supplemented KT 2mg/l and NAA 0.05mg/l.The culture was conducted under controlled temperature(25± 3℃)and light(1 000～2 000 lx,12 hours a day)in the culture room.On the 25th day axil- lary buds germinate,and then clumps with multiple buds are formed from the buds.The clumps subcultured on WB basal medium supplemented with BA 0.5mg/l and NAA 0.025 mg/l for 25 days grow into normal shoots.Rooting ocourres on shoots transferred to the rooting medium containing BA 0.025mg/l,IAA 0.02mg/l and NAA 0.05mg/l.Eighty per- cent of rooted plants are successfully transferred and grow normally in pot-culture soil and under glasshouse conditions.

关 键 词：'美人’梅 茎段 离体培养 

分 类 号：S685.17[农业科学—观赏园艺]