人肝纤维化中肌纤维母细胞的组织原位研究  

IN SITU STUDY OF MYOFIBROBLAST DURING HEPATIC FIBROGENESIS IN HUMAN BEING

作　　者：周侃[1] 翟为溶[1] 庄丽[1] 胡锡琪[1] 

机构地区：[1]上海医科大学病理学教研室,上海静安区中心医院临床免疫室

出　　处：《中国组织化学与细胞化学杂志》1994年第2期134-138,195,共6页Chinese Journal of Histochemistry and Cytochemistry

基　　金：国家自然科学基金

摘　　要：本文以α－平滑肌肌动蛋白（α－ＳＭＡ）作为肌纤维母细胞（ＭＦｂ）的标志，对７３例人肝组织作免疫组化研究，观察α－ＳＭＡ阳性细胞与Ⅰ、Ⅲ型胶原以及炎症细胞的关系。发现在各种慢性肝病的肝组织中α－ＳＭＡ、Ⅰ、Ⅲ型胶原阳性率均明显高于对照组；尤其在慢性活动性病变时，α－ＳＭＡ阳性细胞数与Ⅲ型胶原量均多，而在慢性非活动性病例中Ⅰ型胶原量较多；且各组显示α－ＳＭＡ阳性细胞量的曲线与Ⅲ型胶原基本一致。α－ＳＭＡ阳性细胞的增生及分布与炎症细胞的浸润也密切相关。因此，可籍ＭＦｂ（α－ＳＭＡ阳性）的定位检测来判断人肝纤维化的进展情况。specimens of human liver tissues with different liver diseases were studied immunohistochemically to demonstrate the relationship among α-smooth-muscle-actin(α-SMA),a marker of myofibroblast,collagen typeⅠ、Ⅲ(col Ⅰ、Ⅲ)and inflammatory cells.The results showed that the proliferation and distribution of α-SMA(+)cells were closely related to that of the inflammatory cells.In chronic liver diseases,the amount of α-SMA-positive cells,colⅠand col Ⅲ was much more greater than that in control group. Particularly in the chronic active diseases,the quantity of col Ⅲ was more than that of col Ⅰ as well.However,in chronic nonactie disease. the of colⅠis more than that of col Ⅲ The variations of distibution and the quantity of α-SMA(+)cells among different groups were basically similar to those col Ⅲ.It suggests that detection of MFb,marked with α-SMA in human liver,is a reliable creterion to indicate the progressing of fibrogenesis.

关 键 词：人肝纤维化 肌纤维母细胞 免疫组织化学 胶原 

分 类 号：Q26[生物学—细胞生物学]