BQ＿（123）－多聚赖氨酸携载β－半乳糖苷酶基因向血管平滑肌细胞的靶向导入  

BQ123-POLYLYSINE MEDIATED INTRODUCTION OF pSV-β-LacZ VASCULAR SMOOTH MUSCLE CELLS IN VITRO

作　　者：赵民清 李岱宗[1] 张吉辉[1] 周爱儒[1] 汤健[1] 

机构地区：[1]北京医科大学心血管基础研究所,上海市肿瘤所

出　　处：《北京大学学报（医学版）》1994年第S1期135-138,共4页Journal of Peking University:Health Sciences

摘　　要：本研究连接合成了ＢＱ１２３－多聚赖氨酸（ＢＰ）利用ＢＱ１２３对血管平滑肌细胞（ＶＳＭＣ）膜表面内皮素Ａ型受体（ＥＴＡ）的高度选择性和多聚赖氨酸对ＤＮＡ的强吸附作用，以ＢＰ携载含有β－半乳糖苷酶（β－ＬａｃＺ）基因的表达质粒ｐＳＶ－β－Ｇａｌａｃｔｏｓｉｄａｓｅ（ｐＳＶ－β－ＬａｃＺ）对培养的ＶＳＭＣ和内皮细胞（ＥＣ）转移基因。结果表明，ＢＰ具有向ＶＳＭＣ转基因性能，且依赖与靶细胞表面ＥＴＡ的结合。We constructed a soluble targeted DNA delivery carrier,BQ123-Polylysine,by which plasmid DNA (pSV-β -LacZ)was transfered into vascular smooth muscle cells(VSMC).The expression levels of the re-porter gene(β-LacZ)in treated VSMC and endothelial cells show that BQ123-Polylysine can efficiently trans-fer DNA into VSMC,mediated by endothelin A subtype receptors of VSMC surface.The results suggested that it is possible for investigators to use this delivery carrier as a tool in research VSMC gene regulation and cardiovascular gene therapy.

关 键 词：BQ123-多聚赖氨酸 β-LacZ 血管平滑肌细胞 基因转移 

分 类 号：Q782[生物学—分子生物学]