水稻结瘤共生固氮研究(一)──水稻诱导结瘤和根瘤菌导入  

STUDIES ON RICE'S SYMBIOTIC NITROGEN FIXATON(I)──On Rice's Induced Nodulation and Transfer of the Nodule Bacterium into the plant

作　　者：毛治国[1] 刘志刚[1] 肖庆元[1] 

机构地区：[1]湖南省土壤肥料研究所,长沙410125

出　　处：《生物数学学报》1994年第3期195-200,共6页Journal of Biomathematics

基　　金：总理基金!中的子课题"非豆科作物结瘤固氮研究

摘　　要：一定的水稻品种以无菌水培和砂培，待秧苗长到一定时期，用一定浓度的合成植物生长激素2，4-二氯苯氧乙酸（2.4-D）或二甲四氯钠处理水稻根系，并接种田菁茎瘤根瘤菌（AzorhizobiumCaulinodans），处理后10-15天水稻根系产生瘤状结构（类根瘤），20天后水稻类根瘤经电镜检查，发现根瘤菌已侵入水稻根系，以及根瘤菌在水稻类根瘤内转化形成的类菌体。不同的水稻品种和不同浓度的2，4-D或二甲四氯钠处理，植株的生长状况和根系的结瘤状况各不相同。在参试的11个水稻品种中以湘晚籼4号等四个品种结瘤状况较好。水稻水培2，4-D处理浓度以0.5ppm较为适宜；水稻砂培2，4-D处理浓度以1ppm、二甲四氯钠以2ppm较为适宜．Certain rice varieties,after culturc by stcrilized water or sand,were treated in their seedling's root systems with 2.4-D 2M-4X、MCPA of various concentrations,and inoculated with the nodule bacterium( Azorhizobium Caulinodans).Some nodule-likes,which are structures similar to the nodules,formed in the root system 10--15 days later.And 20 days later the nodule bacterium was found to invade the root system and transform into bacterium--likes under electronic microscope inspection.The plant growth and nodulation varied with the rice varieties and the concentrations of 2.4-D and 2M-4x、MCPA Xiang wan Xian 4(a late rice) and the other three out of the ll varieties tested showed better nodulation.0.5ppm of 2.4-D was available concentration for the water cultured and lppm of 2.4--D or 2ppm of 2M--4x,MCPA were available for the sand cultered.

关 键 词：水稻 根瘤菌 类根瘤 2 4-D 二甲四氯钠 

分 类 号：Q943.2[生物学—植物学]