西红柿叶片SOD基因分离克隆和测序的研究  

MOLECULAR CLONING AND SEQUENCING OF THE CDNA FOR TOMATO SOD

作　　者：王宇光[1] 郭建春[1] 郑学勤[1] 

机构地区：[1]热带作物生物技术国家重点实验室

出　　处：《热带作物学报》1994年第S1期71-74,共4页Chinese Journal of Tropical Crops

摘　　要：利用多聚酶链式反应技术，以西红柿叶片为材料，快速扩增并克隆了Ｓｕｐｅｒｏｘｉｄｅｄｉｓｍ－ｕｔａｓｅ（简称ＳＯＤ）ｃＤＮＡ，并进行序列分析，该基因全长４６５ｂｐ，共编码１５２个氨基酸，与国外已报道基因具有９８％以上的同源性。The cDNA for tomato SOD was cloned from total RNA of young tomato leaf following PCR amplification. Sequencing data proved that the clod cDNA contained an intact region ot tomato SOD gene which was 465bp in length,encoding 152 amino acids.The SOD gene sequence was highlp hom-ologus with the reported sequences.

关 键 词：多聚酸链式反应SOD 基因 序列分析 

分 类 号：S641.2[农业科学—蔬菜学]