等离子点时Mn（Ⅱ）－HSA金属中心的结构  

Structure of the Mn (Ⅱ)-HSA Metal Center at the Isoionic Point

作　　者：杨勇飞[1] 梁宏[1] 俞丽娟 罗锦新[1] 周永洽[1] 

机构地区：[1]广西师范大学化学系,南开大学化学系

出　　处：《广西科学》1994年第2期12-13,59,共3页Guangxi Sciences

基　　金：国家自然科学基金

摘　　要：用紫外光谱研究了等离子点时Ｍｎ（Ⅱ）－ＨＳＡ（Ｈｕｍａｎｓｅｒｕｍａｌｂｕｍｉｎ）金属中心的结构。结果表明：Ｍｎ（Ⅱ）－ＨＳＡ金属中心的配位结构为规则或不规则的四方锥形构型，与生理ｐＨ下存在四方锥→四方平面构型转变不同。Ｍｎ（Ⅱ）的结合位置仍在ＨＳＡ的Ｎ端三肽段，与Ａｓｐ１的α－ＮＨ２，Ｈｉｓ３的咪唑基Ｎ及两个去质子肽氮配位。用配位环境差异可对这两种ｐＨ条件下的不同结果进行解释。The structure of the Mn(Ⅱ)-HSA (Human serum albumin)metal center at the isoionic point have been studied by ultraviolet spectroscopy.It showed that the coordination complex's structure of the metal center was regular or inordinate square-pyramidal configuration, and that differences from the result at physiological pH (squre-pyramidal→square-planar), but the binding site still located at the N-terminal sequence of HSA, and coordinated with α-NH2 of Asp1, imidazolyl of His3 as well as two peptide nitrogens.This could be explained by the discrepancy of the coordinate surroundings.

关 键 词：Mn(Ⅱ)-HSA 等离子点 LMCT谱带 光学电负性 

分 类 号：O562.1[理学—原子与分子物理]