氯化铜对低密度脂蛋白及其脂质成分的影响以及临床实用价值  

The Effect of CuCl_2 on Low Density Lipoprotein(LDL)and Lipid Constituents in LDL and the Clinical Significance

作　　者：张小澍[1] 匡培根[1] 吴卫平 金武道 小野寺庚午 目畤弘文 大池弘三郎 

机构地区：[1]北京海淀区解放军261医院神经科,北京五棵松中国人民解放军总医院神经科,日本弘前大学

出　　处：《卒中与神经疾病》1994年第3期113-115,共3页Stroke and Nervous Diseases

摘　　要：目前认为氧化ＬＤＬ是导致动脉硬化的重要危险因素之一。为研究氧化ＬＤＬ及其脂质的变化，本文作者将１１例健康人的ＬＤＬ置于５μＭＣｕＣｌ_２，溶液中透析，并用ＴＬＣ观察脂质成分的变化。结果为ＣＥ、ＴＧ随透析时间的延长而降低，ＦＦＡ、ＦＣ及ＰＬ则随透析时间的延长而升高，并在透析后２４小时出现明显的、代表过氧化ＣＥ的异常着色点Ｘ_１及Ｘ_２点。由于该实验方法的条件易于控制，重复性好，故为选择性测定过氧化ＣＥ的良好方法，可用于临床研究，分析ＣＶＤ时氧化ＬＤＬ中脂类及过氧化脂质的变化。此外尚可用于研究抗氧化ＬＤＬ及抗过氧化脂质药物。It has be considered that oxidized LDL is one of the important risk factors of atherosclerosis.For researching the oxidized LDL and the changes of the lipids in the oxidized LDL,the LDL from 11 healthy persons was dialyzed into 5 μM CuCl2 solution and changes of the lipid constituents were analyzed on TLC. The results showed that CE and TG decreased with the time of the dialysis prolonged,while FFA,FC and PL increased. The spot X1 and X2 which represented the peroxidized CE were clearly found after the dialysis for 24 hours. Because the experiment is easy to be controlled and reproducible,it is a good method for assaying peroxidized CE selecetively and is useful for clinical studies to analyze the changes of lipid constituents and peroxidized lipids in oxidized LDL in cerebrovascular diseases.

关 键 词：动脉硬化 氧化LDL CuCl₂ 过氧化脂质 

分 类 号：R972.6[医药卫生—药品]