实验性动脉血栓形成TXB_2和6-keto-PGF_(1α)的动态研究  

Study on Change of Level of TXB_2 and PGF_(1α) in Experimental Arterial Thrombosis

作　　者：康平[1] 李叔庚[1] 

机构地区：[1]湖南医科大学,湖南410078

出　　处：《血栓与止血学》1994年第1期2-6,共5页Chinese Journal of Thrombosis and Hemostasis

摘　　要：用聚乙烯导管插管损伤兔主动脉以造成血栓形成,测定血栓形成不同时间TXB_2和6-keto-PGF_(1α)的动态变化。24只家免,分别于插管前、插管后30＇、60＇、120＇采血4次,并分为四组:A组-插管前;B组-插管后30＇;C组-插管后60＇;D组插管后120＇。其中16只家兔进行TXB_2和6-keto-PGF_(1α)及其比值的测定,8只进行血小板聚集度测定。结果是:B、C、D三组的6-keto-PGF_(1α)/TXB_2均明显低于A组(P<0.01),D组明显低于B组(P<0.01);血小板聚集度随着时间延长而逐渐增加。超微结构显示血小板出现明显伪足、肿张、内含大量空泡,失去其内部结构,与纤维蛋白一起附着于去内皮区.血管部分内皮断裂、脱落、暴露出内皮下组织.内弹力膜高度弯曲。本文提供了一种实用有效的实验性动脉血栓形成模型。为早期检测动脉血栓形成提供了重要的实验指标。Rabbit aorta was injured by inserting a polyethylene catheter into femoral artery, and its tip was advanced to aortic arch to cause experimental arterial thrombosis. After having fixed with 4% paraformaldehyde for 24 hours, several pieces of aorta were resected and observed by electron microscopy. In the mean time, blood was collected from carotid artery before, 30 min. 60 min, 120 min after the insertion to measure platelet counts, maximum platelet aggregation. blood levels of TXB2 and 6-keto-PGF1α. The results showed that some deendothelialized areas were observed on the vessel wall and thrombi were formed on these areas. Platelets showed swelling, degranulation. loss of internal structures and idhered to the luminal surface. Platelet counts and maximum platelet aggregation were both higher than those before the insertion. They increased with the time after the insertion. 6-keto-PGF1α decreased from pre-insertion level of 640. 02±42. 67pg/ml to post-insertion levels of 223. 51±25. 34pg/ml at 30 min, 144. 67±17. 24pg/ml at 60 min, 113. 93±11. 36pg/ml at 120 min. Before insertion. TXB2 was 211. 36 ± 23. 42pg/ml. After insertion at 30 min, 60 min, 120 min, TXB2 increased to 681. 64 ± 49. 73pg/ml, 1114. 51 ± 62. 57pg/ml, 1343. 23±68. 34pg/ml. So 6-keto-PGF1α/TXB2 ratio reduced significantly(P<0. 01). Their values were 3. 03±0. 12, 0. 34±0. 03, 0. 13 ± 0. 02, 0. 08±0. 01, respectively.The study have provided experimental data for basic and clinic research of arterial thrombosis.

关 键 词：血栓形成 6-keto-PGF₁α) TXB₂ 血小板聚集度 超微结构 兔 

分 类 号：R543[医药卫生—心血管疾病]