Relationship Between Electron Transport or Proton Transduction and ATP Synthesis in Fusion Membranes in Light  

作　　者：李淑俊 王健 徐亚南 邵菁 彭建新 

机构地区：[1]Shanghai Institute of Plant Physiology, Academia Sinica, Shanghai 200032, PRC

出　　处：《Science China Chemistry》1993年第12期1490-1500,共11页中国科学（化学英文版）

基　　金：Project supported by the National Natural Science Foundation of China.

摘　　要：The phosphorylation coupling factors of the chloroplast thylakoid membranes were plucked off to form deficient membranes which were combined with crista membranes to form a fusion system. This membranes reconstituted ATP synthesis activity in light. However, the △pH in the reaction medium was not observed.This paper is a report on some of the results obtained recently in connection with the problem. Various examinations and measurements were used and this phenomenon was repeated. Through the reconstituted system, (thylakoid membranes combined with crista membranes) the kinetic proton changes were studied. It proved that the photophosphorylation activity of this system increased with the increase of the quantity of the combined crista membranes to which, however, the △pH in medium, the effects of 9-AA fluorescence quenching, and the absorption change of neutral red in the membrane pockets were inversely proportional. For this phenomenon, analyses and discussions on the relationship between electron transport or proton transduction and ATP synthesis in the fusion membranes have been made.The phosphorylation coupling factors of the chloroplast thylakoid membranes were plucked off to form deficient membranes which were combined with crista membranes to form a fusion system. This membranes reconstituted ATP synthesis activity in light. However, the △pH in the reaction medium was not observed.This paper is a report on some of the results obtained recently in connection with the problem. Various examinations and measurements were used and this phenomenon was repeated. Through the reconstituted system, (thylakoid membranes combined with crista membranes) the kinetic proton changes were studied. It proved that the photophosphorylation activity of this system increased with the increase of the quantity of the combined crista membranes to which, however, the △pH in medium, the effects of 9-AA fluorescence quenching, and the absorption change of neutral red in the membrane pockets were inversely proportional. For this phenomenon, analyses and discussions on the relationship between electron

关 键 词：THYLAKOID membranes DEFICIENT membranes CRISTA membranes FUSION membranes PROTON TRANSDUCTION with ATP synthesis. 

分 类 号：O6[理学—化学]