晚期日本血吸虫病患者血清可溶性白细胞介素-2受体的研究  

SERUM SOLUBLE INTERLEUKIN-2 (IL-2) RECEPTOR LEVELS IN PATIENTS WITH LATE SCHISTOSOMIASIS JAPONICA

作　　者：张立煌[1] 孙永良[1] 蔡卫民 方海林[1] 姚航平[1] 刘荣华[1] 林舜华[1] 何南祥[1] 

机构地区：[1]浙江医科大学传染病研究所,杭州310003

出　　处：《中国血吸虫病防治杂志》1993年第4期206-208,共3页Chinese Journal of Schistosomiasis Control

摘　　要：采用双抗体夹心ELISA法,检测47例晚期日本血吸虫病患者血清可溶性白细胞介素-2受体(sIL-2R),同时用间接免疫荧光法测定其外周血单个核细胞(PBMCs)膜IL-2受体(mIl-2R)的表达水平。结果表明晚血患者血清sIL-2R较正常人明显增高,而mIl-2R表达明显低下(P<0.01),其sIL-2R水平与mIl-2R表达呈负相关(r=-0`62,P<0.01).血清sIL-2R高低与COPT阳性与否无关,说明患者血清sIL-2R高低与病原体本身无直接关系,而与患者是否伴腹水有关,提示sIL-2R水平与疾病进展程度有关。观察患者血清sIL-2R的高低可作为疾病病程的监测指标。The survey of the soluble IL-2 receptor (sIL-2R) level in the sera and the membrane IL-2 receptor (mIL-2R) expression on the peripheral blood mononuclear cells (PBMCs) from 47 cases of late schistosomiasis japonica was reported. The measurement for sIL-2R was done with the double antibody sandwich ELISA. Indirect immunflurescence was performed in the measurement for mIL-2R. The levels of sIL-2R in sera from 47 patients with late schistoso-miasis was found to be higher than that in control (P<0. 01) ; while the mIL-2R on PBMCs, was lower in comparing with control (P<0. 01). The negative correlation was found between the levels of sIL-2R in sera and the expression of mIL-2R on PBMCs in these patients (r= -0. 62. P<0. 01). That no relationship exists between the levels of sIL-2R and the result of COPT suggests that high levels of sIL-2R might not be due to the direct effect of parasite itself. Therefore late schistosomiasis should be recognized as an immune disorder, and the levels of sIL-2R were related to ascites. It is suggested that sIL-2R could be considered as an efficient indicator for surveillance of schistosomiasis.

关 键 词：日本血吸虫病 血清可溶性白细胞介素-2受体 外周血单个核细胞膜IL-2受体 ELISA IFAT COPT 

分 类 号：R532.21[医药卫生—内科学]