Structure effect of nucleotides in terbium(Ⅲ)-nucleotide fluorescent reaction—-New evidence for the binding sites of terbium(Ⅲ) on nucleotides  

作　　者：LI Yuan-Zong CHANG Wen-Bao CI Yun-Xiang 

机构地区：[1]Department of Chemistry,Beijing University,Beijing 100871

出　　处：《Chinese Journal of Chemistry》1993年第6期524-531,共8页中国化学(英文版)

基　　金：Work supported by the National Natural Science Foundation of China

摘　　要：Upon addition of Tb^(3+) to 16 nucleotides and homopolynucleotides, all of them showed a characteristic green emission from Tb^(3+), but with much different intensity, upon excitation in the aromatic region of bases. The result suggested that nucleotides with at least one carbonyl group in nucleotide bases are better enhancers to the fluorescence of Tb3+. The complexes of ATP, GDP and GTP with T5^(3+) are synthesized as two types of models. Guanine tpye nucleotides with one carbonyl group in the bases are the best enhancers, while adenine type nucleotides with no carbonyl group in the bases are poorest enhancers to the fluorescence of Tb^(3+). Comparing the IR spectra of ATP, GTP, GDP and their Tb^(3+) complexes suggested that C-6 carbonyl group in GTP and GDP may be involved in complex formation, which may be responsible for the effective energy transfer. This is further supported by comparing the UV spectra of ATP, Poly(A), GTP, and Poly(G) with their Tb^(3+) complexes in water solution.Upon addition of Tb^(3+) to 16 nucleotides and homopolynucleotides, all of them showed a characteristic green emission from Tb^(3+), but with much different intensity, upon excitation in the aromatic region of bases. The result suggested that nucleotides with at least one carbonyl group in nucleotide bases are better enhancers to the fluorescence of Tb3+. The complexes of ATP, GDP and GTP with T5^(3+) are synthesized as two types of models. Guanine tpye nucleotides with one carbonyl group in the bases are the best enhancers, while adenine type nucleotides with no carbonyl group in the bases are poorest enhancers to the fluorescence of Tb^(3+). Comparing the IR spectra of ATP, GTP, GDP and their Tb^(3+) complexes suggested that C-6 carbonyl group in GTP and GDP may be involved in complex formation, which may be responsible for the effective energy transfer. This is further supported by comparing the UV spectra of ATP, Poly(A), GTP, and Poly(G) with their Tb^(3+) complexes in water solution.

关 键 词：nucleotide fluorescent reaction Structure effect of nucleotides in terbium New evidence for the binding sites of terbium 

分 类 号：O6[理学—化学]