镉对大鼠心肌细胞毒作用的实验研究  

EXPERIMENTAL STUDY OF THE TOXIC EFFECTS OF CADMIUM ON CARDIOMYOCYTES IN RATS

作　　者：王瑞绵 常青 戎诚兴 孟扬 王福元 周玲宝 徐亚明 

机构地区：[1]湖北医科大学生理学教研室,武汉,430071 [2]湖北医科大学组织学与胚胎学教研室 [3]湖北医科大学卫生学教研室

出　　处：《中国组织化学与细胞化学杂志》1993年第2期124-129,181,共7页Chinese Journal of Histochemistry and Cytochemistry

摘　　要：本文用组织化学、电镜、能谱分析及血镉测定等方法研究了低浓度镉对大鼠心肌细胞的毒作用。大鼠经饲料染镉12周,血镉浓度达5.42±0.65μg/L(对照组为1.01±0.38μg/L)。染镉组大鼠心肌细胞出现镉的频谱峰,LDH活性下降,但SDH活性增强,图像分析显示该两种酶活性的改变皆与各自的对照组差异有非常显著性意义(P<0.001)。肌球蛋白ATP酶活性也较对照组有所下降。电镜下,染镉大鼠心肌细胞部分肌节变短,肌丝紊乱、断裂、密度降低,失去A及I带的排列规则,Z盘增宽。线粒体成堆地聚集在肌丝区之间或肌膜下,部分出现肿胀、嵴断裂、甚至变成含有絮状物的大泡。以上结果提示低浓度镉对大鼠心肌细胞有毒作用。The toxic effects of Cd in low concentration on cardiomyocytes in rats were studied by using methods of histochemistry, electron microscopy, energy spectrum analysis and determination of blood Cd. The rats in Cd group were fed with CdCl_2 mixed in rodent chow for 12 weeks. At the end of 12 weeks, the average concentration of blood Cd in Cd group was 5.42±0.65μg/L(1.01±0.38μg/L in control); the frequency spectrum peak of Cd appeared in cardiomyocytes; the LDH activity of cardiomyocytes decreased, while the SDH activity increased. The image analysis showed that the activities of LDH and SDH were significantly different from those of controls respectively(P<0.001). The activity of myosin ATPase decreased as compared with control gronp. Under electron microscope, it appeared that in a few cardiomyocytes some sarcomeres became shortened, the myofilaments disarranged or broken and their normal density. The Z disc thickened in these sarcomeres. The mitochondria piled up between the myofilamental areas or beneath the sarcolemma. Some mitochondria were swollen, or their cristae disrupted. even changed into vesicles containing cotton—like substance. The results mentioned above indicate that the Cd has damaged the cardiomyocytes of rats.

关 键 词：镉 心肌细胞 组织化学 超微结构 能谱 血液 大鼠 

分 类 号：Q[生物学]