融合热释放对低温冷冻人骨髓细胞的核酸与酶类活性的影响  

INFLUENCE OF FUSIN HEAT RELEASE ON ACTIVATION OF NUCLEIC ACID AND ENZYMES IN CRYOPRESERVATED HUMAN MARROW CELLS

作　　者：高玉民[1] 梁丽华[1] 谢艳[1] 马久才[1] 

机构地区：[1]空军总医院血液病学实验室,北京100036

出　　处：《中国组织化学与细胞化学杂志》1993年第4期333-336,共4页Chinese Journal of Histochemistry and Cytochemistry

摘　　要：本文用定性定量组织细胞化学方法,对冻前及不同降温条件冻存的人骨髓细胞的 DNA、碱性磷酸(ALP)、过氧化物酶(POX)活性进行测定。在程序降温过程中,没有消除融合热的骨髓细胞的 DNA、ALP、POX 活性均显著下降。消除融合热后,冻存骨髓细胞 DNA 活性没有改变,ALP、POX 活性略有下降。因而,融合热的释放是骨髓细胞生物活性下降的重要因素。Activation of deoxyribonucleic acid(DNA),alkaline phosphatase(ALP)and peroxidase (POX)of human marrow cells were measured under prefreezing and different freezing condi- tions by using quantitative and qualitative histochemistry method.In the course of ordinary freezing,the activation of DNA.ALP and POX in human marrow cells were remarkably re- duced when fusion heat of freezing bone marrow cells wasnlt removed.After removing of fu- sion heat of freezing bone marrow cells the activation of DNA in cryopreservated human mar- row cells remained unchanged but that of ALP and POX in then reduced a little reduce. These results showed that the fusion heat release of cryopreservated marrow cells was a ma- jor reason for the reduction of biological activation in marrow cells.

关 键 词：融合热 低温冷冻 骨髓细胞核 酸与酶活性 

分 类 号：Q[生物学]