庆大霉素/水杨酸/壳聚糖复方纳米粒的制备及体外释放  

Preparation of the gentamicin and salicylic acid-loaded chitosan nanoparticles and its in vitro releasing investigation

作　　者：季金苟[1] 郝石磊[1] 刘玮琦[1] 吴丹君[1] 徐溢[1] 陈际达[1] 

机构地区：[1]重庆大学化学化工学院,重庆400030

出　　处：《重庆大学学报（自然科学版）》2011年第11期100-104,共5页Journal of Chongqing University

基　　金：重庆市自然科学基金资助项目(CSTC2008BB4056);重庆大学研究生科技创新基金资助项目(201005A1A0010333);重庆大学211工程三期创新人才培养计划建设项目(S-09103)

摘　　要：通过壳聚糖与三聚磷酸钠的离子交联作用制备了具有拮抗庆大霉素耳毒性功效的庆大霉素/水杨酸复方壳聚糖纳米粒。用紫外分光光度计、纳米粒度仪和Zeta电位仪、透射电子显微镜、扫描电子显微镜、傅里叶变换红外光谱仪和X射线衍射仪等考察了壳聚糖纳米粒的粒径、zeta电位、形态、载药能力及体外释放行为。结果显示复方纳米粒为球形,平均粒径为40nm;庆大霉素与水杨酸的包封率分别为(91.24±0.24)%和(80.75±0.15)%,载药量分别为(34.15±1.02)%和(38.35±0.48)%;在体外释放试验中,庆大霉素和水杨酸的释放都符合双指数动力学方程,实现了两者同时缓慢释放。这预示复方纳米粒可能具有拮抗庆大霉素耳毒性的功效,同时纳米粒的缓释效果也有望降低庆大霉素肾毒性的产生。Gentamycin（GM） and salicylic acid（SA） loaded chitosan（CS） nanoparticles are prepared and used to inhibit the ototoxicity of GM.Nanoparticles are prepared via the cross-linking method by CS and sodium tripolyphosphate.The characteristics of nanoparticles such as size,zeta potential,shape,loading capacity,and in vitro release profiles are determined.The nanoparticles are spherical in shape with an average diameter of 40 nm.The entrapment efficient of GM and SA is（91.24±0.24）% and（80.75±0.15）% respectively,and loading capacity are（34.15±1.02）% and（38.35±0.48）%.The drug release shows good sustained-release effect and follows ambiexponent kinetic equation.It demonstrates that GM and SA loaded CS nanoparticles have promising potential effect on antagonism ototoxicity of GM,and the nephrotoxicity may be also decreased through the expected sustained-release characteristics of CS nanoparticles.

关 键 词：庆大霉素 耳毒性 水杨酸 壳聚糖 纳米粒 

分 类 号：R978.1[医药卫生—药品]