REA对小麦花药培养的影响  

The Effect of REA on Wheat Anther Culture

作　　者：和现昌[1] 王金兰[1] 海燕[1] 徐向阳[1] 

机构地区：[1]河南省农科院小麦研究所,郑州450002

出　　处：《华北农学报》1993年第S1期54-59,共6页Acta Agriculturae Boreali-Sinica

摘　　要：研究了REA对小麦花药培养的影响,结果表明,REA对愈伤组织诱导率、绿苗诱导率的提高均有显著作用.使不同基因型的愈伤组织在C_(17)培养基上的诱导率提高了1.47～12.25倍,在“癸”培养基上提高了0.52～3.40倍;基因型、培养基和REA之间存在着互作,且对不同的基因型REA的最适附加量不同;对愈伤组织的生长有促进作用;可促进绿苗分化,大幅度提高绿苗分化率;并可降低白苗诱导率.This-study was conducted to identify the effect of REA on wheat anther culture with 8 genotypes and 3 media in 1991-1992. Results obtained were as follows; (1) REA has significant effects on callus induction frequency. In the presence of suitable amounts of REA, the callus induction frequencies of the genotypes cultured on C17 medium were 2. 47-13. 25 times as high as those without REA. When REA was added to KUI medium, the callus induction frequencies increased by 52-340%. For most of the genotypes used, it's useful for improving callus frequency to add 3-6mg/L REA to C17 medium and KUI medium. (2) The interaction of REA, media and genotypes was found, the most suitable addition amount of REA was relative to genotypes and media. (3) REA could promote the growth of plantlets and increase the green plantlet frequency at great degree. It's very useful for improving green plantlet frequency to add 6mg/L REA to MS medium. (4) REA could decrease albino frequency. It was suggested that albino was relative to nutrition conditions. The production of albino could be partly controllcd by means of adjusting the ingredients of the medium.

关 键 词：小麦 花药培养 REA附加剂 愈伤组织 绿苗 白苗 诱导率 

分 类 号：S3[农业科学—农艺学]