The technics of breeding a new transgenic tobacco cultivar resistant to mosaic diseases and its extention  

作　　者：周汝鸿 张振臣 吴青 孙清 方荣祥 莽克强 田颖川 王桂玲 侯文华 李克治 董继超 杨静 

出　　处：《华北农学报》1993年第S2期58-63,共6页Acta Agriculturae Boreali-Sinica

摘　　要：This paper reports a new transgenic tobacco line NC89 resistant to both tobaccomosaic virus(TMV)and cucumber mosaic virus(CMV).It deals with the details of field experi-ments of the whole process of segregation and selection of the progenies of transgenic plant popu-lation.By using the gene transfer technics,some transgenic plants resistant to mosaic disease froma flue-cured tobacco cultivar NC89 were obtained in green house in 1989.Thereafter,throughfield inoculation and selection from the 2nd and 3rd generations,a homozygous line resistant toboth viruses and with good qualities was bred out.The inheretant stability of the resistance of thenew line was confirmed through the 4th to 6th generations.In 1992,the transgenic resistant linewas planted about 7000 hectares in Henan province.The relative effect of controlling mosaic dis-eases of tobacco was 50—70%.The out-put value increased 30—50% per unit area.This is a newexample of growing a transgenic virus-resistant Plant in large scale in China.This paper reports a new transgenic tobacco line NC89 resistant to both tobacco mosaic virus (TMV)and cucumber mosaic virus (CMV). It deals with the details of field experiments of the whole process of segregation and selection of the progenies of transgenic plant population. By using the gene transfer technics,some transgenic plants resistant to mosaic disease from a flue-cured tobacco cultivar NC89 were obtained in green house in 1989. Thereafter, through field inoculation and selection from the 2nd and 3rd generations, a homozygous line resistant to both viruses and with good qualities was bred out. The inheretant stability of the resistance of the new line was confirmed through the 4th to 6th generations. In 1992,the transgenic resistant line was planted about 7000 hectares in Henan province. The relative effect of controlling mosaic diseases of tobacco was 50 - 70%. The out-put value increased 30-50% per unit area. This is a new example of growing a transgenic virus-resistant plant in large scale in China.

关 键 词：GENE-TRANSFER TOBACCO MOSAIC VIRUS cucumber MOSAIC VIRUS NEW transgenictant line of TOBACCO 

分 类 号：S3[农业科学—农艺学]