自制简易胚胎冷冻器冷冻奶牛胚胎试验  

Experiment of Freezing Bovine Embryos with Self-made Simple Freezer

作　　者：窦忠英[1] 王新庄[1] 樊敬庄[1] 高志敏[1] 徐照学[1] 国永周 韩晓良 李继合 刘恩耀 张强 王效涵 薛帅岐 

机构地区：[1]西北农业大学奶牛胚胎移植试验站,陕西杨陵712100 [2]西安市红庙坡奶牛场 [3]西安市未央区畜牧兽医工作站 [4]武功县武功镇畜牧兽医工作站

出　　处：《西北农林科技大学学报（自然科学版）》1993年第S2期69-72,共4页Journal of Northwest A&F University(Natural Science Edition)

基　　金：农业部生物技术资助项目

摘　　要：用FSH+PGF_(2)超排黑白花奶牛,以非手术方法采得7日龄早期胚胎。应用含10％甘油卵细胞培养液作为胚胎冷冻保护液,采用自制简易胚胎冷冻器冷冻,在-196℃液氮中保存。冻胚在35℃水浴中解冻,应用92.1g/L甘油+171.2g/L蔗糖PBSS,46g/L甘油+171.2g/L蔗糖PBSS和171.2g/L蔗糖PBSS三步脱去甘油,用含20％犊牛血清PBS将胚胎清洗2遍后装管移植。共解冻18枝冻胚,16枚可用。可用胚率为84.2％(16/18)。采用非手术方法移植16头受体牛,5头妊娠产犊,移植妊娠率为31.5％(5/16)。Donor Holsteins were superovulated with Fsh+PGF2, and flushed non-surgically at day 7. Cryoprotectant was cocyte culture medium containing 10% glycerol. Fmbryo freezing was accomplished by means of self-made freezier. The embryos were stored in liquid nitrogen at -196℃. Frozen embryos were thawed in a 35'C water bath, and the cryoprotectant was removed by three steps of 92. 19 g/L glycerol+ 171. 2 g/L sucrose in PBSS,46 g/L glycerol + 171. 2 g/L sucrose in PBSS. and 171. 2 g/L sucrose in PBSS. Embryos were washed twice in 20% PBS and transferred non-surgically into the uterus of recipients. Eighteen frozen embryos were thawed, sixteen of them could be used. The rate of available embryos was 84. 2% (16/18). Available embryos were transferred non -surgically to 16 recipients. Five of them be came pregnant. The transplant pregnancy rate was 31. 3% (5/16).

关 键 词：冷冻胚胎 妊娠率 奶牛 

分 类 号：S[农业科学]