视交叉后区内胆囊收缩素神经元与后叶加圧素神经元之间的相互关系——免疫电镜双标研究  

RELATIONSHIP BETWEEN CCK-AND VP-NEURONS IN THE RETROCHIASMATIC AREA—DOUBLE LABELED IMMUNOELECTRON MICROSCOPIC STUDY

作　　者：朱长庚[1] 刘汉涛[1] 张喜京[1] 

机构地区：[1]同济医科大学解剖学教研室,武汉430030

出　　处：《解剖学报》1991年第S1期1-6,131,共7页Acta Anatomica Sinica

基　　金：国家自然科学基金

摘　　要：应用包埋前(PAP)免疫电镜双标技术对大鼠下丘脑视交叉后区内胆囊收缩素(CCK)神经元和后叶加压素神经元的相互关系进行了超微结构研究.先用DAB法显示VP免疫反应,充分洗涤后,再用钼酸铵-TMB法显示CCK免疫反应,经DAB-氯化钴稳定后作环氧树脂包埋。电镜观察发现:在视交叉后区内,VP样免疫反应产物呈电子密度高的颗粒状或絮状沉淀,弥漫分布.CCK样免疫反应产物则为高电子密度的针状或块状,散在分布.VP样胞体较小,呈卵圆形。CCK样胞体为中等大,呈多角形.CCK样胞体和树突可由非CCK和非VP轴突末梢接受传入性突触.VP样轴突可由VP或非VP轴突末梢接受传入性突触。特别值得注意的是,CCK样轴突末梢可与VP样轴突形成传出性轴轴突触:反之,VP样轴突也可与CCK样轴突建立传出性轴轴突触.以上结果首次证明:在视交叉后区不仅存在着CCK和VP神经元,而且,这两种肽能神经元之间存在着相互调控的突触联系,这为进一步阐明下丘脑神经内分泌功能的某些调控机制提供了新的超微结构依据。The relationship between CCK-and VP-neurons in the rat retrochiasmatic area was studied at ultrastructural level by means of pre-embedding(PAP) double immunoelectron microscopic labeling technique. First, the VP-immunoreactivity was demonstrated by DAB method. After thoroughly washing, the CCK-immuno- reactivity was revealed by ammonium molybdate-TMB method. Being stabili- zed by DAB-cobalt chloride, the sections were embedded in Epon 812. Under ele- ctron microscope, it was observed that in the retrochiasmatic area, the VP-LI products distributed diffusely as high electron dense granular or flocculent depo- sits, whereas the CCK-LI products distributed sparsely as needle-or mass-like deposits. VP-LI perikarya were small in size with oval shape and CCK-LI peri- karya were medium in size with polygonal shape. CCK-LI perikarya and dendri- tes received afferent synapses from non-CCK- and non-VP-axonal terminals VP- LI axons received afferent synapses from VP and non-VP-axonal terminals It was interesting that the VP-LI axonal terminals formed efferent axoaxonic syna- pses with CCK-LI axonal endings and, vice versa, the CCK-LI axonal terminals established also efferent axoaxonic synapses with VP-LI axonal endings. The above mentioned results identified for the first time that in the rat retrochiasma- tic area not only there were CCK- and VP-neurons, but also there were reciprocal synaptic regulations between above two kinds of peptidergic neuron, providing new ultrastructural basis for the regulatory mechanism of the neuroendocrine in hypothalamus.

关 键 词：视交叉后区 胆囊收缩素 后叶加压素 免疫电镜 双重标记 

分 类 号：R322[医药卫生—人体解剖和组织胚胎学]