显示分化阶段不同的细胞的荧光染色法  

FLUORESCENT STAINING METHOD DEMONSTRATING CELLS IN DIFFERENT STAGES OF DIFFERENTIATION

作　　者：许屏[1] 梁玉[1] 任怡敏[1] 白岚 

机构地区：[1]天津第二医学院组织胚胎学教研室,天津300203 [2]天津第二医学院附属医院内科,天津300203

出　　处：《解剖学报》1991年第S1期50-54,156,共6页Acta Anatomica Sinica

摘　　要：荧光染色法是一种敏感的细胞化学染色法,它可以反映出细胞生物化学成分的微小变化。用硫代黄素荧光染料配制的7690-Xu荧光染液可以使处于分化阶段不同的细胞呈现异质性荧光。分化程度低的幼稚细胞呈蓝色荧光,分化程度越低,蓝色荧光越深。随着细胞的分化,细胞的蓝色荧光逐渐向光谱的红色光侧偏移.细胞质的蓝色荧光逐渐变为淡蓝色、淡蓝绿色,最后呈黄色。细胞核的蓝色荧光亦经灰蓝色变为桔黄色或桔红色。分化程度高的各种器官的细胞胞质均为黄色,核为桔黄-桔红色.Fluorescent staining method is a sensitive cytochemical staining method, it can reflect minimal changes of biochemical components of cells. The 7690-Xu fluorescent staining solution prepared with thioflavine can show the heterogeneo- us fluorescence of cells in different stages of differentiation. The younger cells with low degree of differentiation show blue fluorescence, the lower the degree the deeper the color. Along with the differentiation of cells, the blue color of fluorescence deflects to the red color of spectrum gradually, the blue color of fluorescence of cytoplasm changes gradually to bluish, bluish-green, and finally yellow color. The blue color of fluorescence of nucleus changes through greyish- blue to orangeyellow or orange-red.The cytoplasm of cells in various organs with high degree of differentiation appears yellow, and the nucleus orange-yellow or orange-red in color.

关 键 词：硫代黄素 7690—Xu 荧光染液 异质性荧光 胸腺细胞 淋巴细胞 巨噬细胞 生精细胞 

分 类 号：R322[医药卫生—人体解剖和组织胚胎学]