党参益智作用研究  

NOOTROPIC EFFECT OF DANGSHENG, CODONOPSIS PILOSULA (FRANCH.) NANNF

作　　者：张磊[1] 刘干中[1] 

机构地区：[1]中日友好医院临床医学研究所药物药理室,北京100013

出　　处：《中药药理与临床》1990年第6期9-13,共5页Pharmacology and Clinics of Chinese Materia Medica

摘　　要：用小鼠跳台法和大鼠操作式条件反应——固定比率操作观察了党参20％乙醇提取物对学习记忆的影响,结果表明党参可拮抗东莨菪碱造成的小鼠记忆获得不良,改善亚硝酸钠造成的小鼠记忆巩固障碍及40％乙醇造成的小鼠记忆再现缺损,部分对抗东莨菪碱对大鼠固定比率操作的抑制。党参进一步分离的正丁醇部分可以重现上述作用。党参正丁醇部分对M胆碱能受体无亲和力,但可使中枢M胆碱受体密度显著增高。但它既不能对抗东莨菪碱所致小鼠大脑皮层及海马Ach含量的降低,其本身也不能提高皮层和海马的Ach含量。以上结果提示党参对学习记忆确有改善作用,胆碱能通路可能是其发生作用的一个重要方面。Twenty Percent alcohol extract of Dangshen (DS-A) was used for facilitation of memory tested by one trail passive avoidance response (step down test) in mice and operant conditional reflex (fixed ratio) in rats. The extract reduced the impairment of acquisition of memory produced by scopolamine (SCPL), the disruption of consolidation of memory caused by sodium nitrite and the deficit of retrieval of memory induced by alcohol. And also, DS-A could improve operant conditional reflex impaired by SCPL. Similar effects were observed with the n-butyl alcohol extrect (DS-FIII), further purified from DS-A, of the drug. It suggested that the active components of Dangshen may be in DS-FIII. Effect of DS-FIII on M-cholinergic receptors were measured by radioligand receptor assay (RRA).No specific binding; of DS-FIII to M-receptor was observed. However, 5 days after ip injection of DS-FIII higher Bmax values were observed. Effects of DS-FIII and SCPL on Ach content of cortex and hippocampus were measured by radioimmunoassay(RIA) .SCPL reduced the Ach content in cortex and hippocampus markedly. Whereas, DS-FIII could not affect the reduction and raise the Ach content. The results suggested that one of mechanisms of action of Dangshen on memory facilitation may be related to cholinergic system. But, the mechanism of the nootropic action of Dangshen is very complicate and need to be further studied.

关 键 词：党参 学习和记忆 AchM胆碱受体 

分 类 号：R28[医药卫生—中药学]