TRANSCRIPTION AND TRANSLATION OF ALFALFA LEGHEMOGLOBIN GENES DURING ROOT NODULE DEVELOPMENT  

作　　者：荆玉祥 ALAN S.PAAU WINSTON J.BRILL 

机构地区：[1]Institute of Botany, Chinese Academy of Sciences, Beijing 100044, PRC [2]Department of Bacteriology and Center for Studies of Nitrogen Fixation, University of Wisconsin, Madison, WI 53562, USA Agracetus,8520 University Green,Middleton,WI 53562,USA

出　　处：《Science China Chemistry》1990年第9期1078-1083,共6页中国科学（化学英文版）

摘　　要：LegHb mRNAs isolated from 2 to 10-week old alfalfa nodules are actively translated in vitro to give a full component of legHb products suggesting that legHb genes, once expressed, are not regulated at the transcription level during the lifetime of a nodule. In vitro translation studies with legHb mRNAs from 10-week old nodules indicate that translation of mRNAs is partially inhibited by addition of alfalfa legHb components themselves and is not promoted by addition of myoglobin and hemoglobin as compared with those of alfalfa legHb mRNAs isolated from 4-week old nodules. This intimates that the regulation of alfalfa legHb synthesis in old nodules at the translation level is weakened.LegHb mRNAs isolated from 2 to 10-week old alfalfa nodules are actively translated in vitro to give a full component of legHb products suggesting that legHb genes, once expressed, are not regulated at the transcription level during the lifetime of a nodule. In vitro translation studies with legHb mRNAs from 10-week old nodules indicate that translation of mRNAs is partially inhibited by addition of alfalfa legHb components themselves and is not promoted by addition of myoglobin and hemoglobin as compared with those of alfalfa legHb mRNAs isolated from 4-week old nodules. This intimates that the regulation of alfalfa legHb synthesis in old nodules at the translation level is weakened.

关 键 词：LEGHEMOGLOBIN root NODULES transeription translation MEDICAGO SATIVA L. Vernal 

分 类 号：O6[理学—化学]