一种改良的Dot-ELISA法  

A MODIFIED DOT-ELISA

作　　者：薛海筹[1] 裘丽姝[1] 朱震霞[1] 张永红[1] 

机构地区：[1]中国预防医学科学院寄生虫病研究所

出　　处：《中国血吸虫病防治杂志》1990年第2期35-38,共4页Chinese Journal of Schistosomiasis Control

摘　　要：改良的Dot-ELISA法,即将点好抗原的NC膜固着于PVC浅孔软板内进行试验。本文以血吸虫卵抗原、肺吸虫成虫抗原、华枝睾吸虫成虫抗原、细粒棘球蚴囊液抗原等4种抗原膜,用改良的Dot-ELISA法,对血吸虫病、肺吸虫病、华枝睾吸虫病、细粒棘球蚴病患者血清各15例以及正常人血清33份进行试验。结果表明:各种抗原与其对应的血清反应在适宜的比例中阳性率可达93％～100％。假阳性率3％。这种改良的Dot-ELISA法,简便易行,可望发展成为一种适用于现场开展多种寄生虫调查的药盒。The principles of the modified Dot-ELISA are to render the antigen dotted Nc paper adherent to the bottom of the shallow wells (10mm x 1.5mm) on a PVC plate and to apply antigens of 4 species of parasite i.e. Schistosoma japonicum, Paragonimus westermani, Clonorchis sinensis and Echinococcus granulosus separately on the same peice of NC paper, which is clearly partitioned into 4 squares by a cross( + ). Hence one serum sample can be screened against 4 antigens concurrently, and reactions with homologus and heterologus antigens can be compared simultaneousely. Sixty serum samples, 15 for each parasitosis collected from parasitologically comfirmed patients,were tested by using this modified Dot-ELISA. 33 sera from healthy persons were employed as controls. The results showed that at optimum dilutions, the positive rate for the homologous antigen-antibody reaction reached 93%~100%, while false positive rate being 3%. With heterologous antibodies,cross reactions of 0~20% were observed.The modified Dot-ELISA turned out to be more convenient, labor-saving , easy to perform and could be developed into a diagnostic kit for multiple parasitological mass survey.

关 键 词：华枝睾吸虫 细粒棘球蚴病 肺吸虫病 棘球蚴囊液抗原 抗原抗体 血清反应 DOT-ELISA 软板 假阳性 包虫病 

分 类 号：R532.21[医药卫生—内科学]