应用核酸斑点杂交法检查正常分娩期妇女尿中人巨细胞病毒DNA  

SURVEY 0F URINE SPECIMENS FROM WOMAN AT THE TlME 0F DELIVERY IN SHENYANG FOR CYTOMEGALOVIRUS INFE-CTI0N BY MEANS 0F DNA-DNA HYBRIDIZATI0N TEST

作　　者：谢光临[1] 那立新[1] 王福忠[1] 杨明久[1] 

机构地区：[1]中国医科大学免疫研究室

出　　处：《微生物学杂志》1990年第Z1期55-57,共3页Journal of Microbiology

摘　　要：用α-^(32)P-dcTP标记人巨细胞病毒(Cytomegalovirus Human type,简称HCMV)DNA片段做探针,用核酸杂交方法检测CMV DNA,其敏感性达Pg水平。并抽样检测了沈阳地区分娩期妇女尿标本。在25份标本中查出HCMV DNA阳性者占6份,排毒率24％,并对实验技术及实验结果进行了讨论。HCMV Were examined by using DNA hybridization test with cloned HCMV DNA probe. The sensitivity arrives at pg level. 25 urine specimens from woman at the time of delivery in shenyang were examined. 6 out of 25 samples showed positive result. The positive rate is 24 per cent. The DNA—DNA hybridization test is more rapid, sensitive and specific than the CMV isolation and examining CMV antibody. The method is useful tool for researching CMV.

关 键 词：DNA 斑点杂交 人巨细胞病毒 正常分娩 尿标本 同位素掺入 梯度稀释 特异性 亚临床感染 实验技术 

分 类 号：Q93[生物学—微生物学]