大豆花叶病毒(SMV)提纯方法的比较研究  

STUDIES ON THE COMPARISON OF THREE METHODS FOR PURIFICATION OF SOYBEAN MOSAIC VIRUS

作　　者：李尉民 濮祖芹 

机构地区：[1]南京农业大学植物保护学系

出　　处：《南京农业大学学报》1990年第S1期56-59,共4页Journal of Nanjing Agricultural University

摘　　要：用差速离心和蔗糖垫离心,仿 Soong 和 Milbrath 的方法提纯 SMV,病毒得率分别为24.5,47.1ms/kg 病叶.用作者的方法提纯 SMV 的产量可达199.2mg/kg 病叶,所得病毒粒体具有生物活性,免疫兔子得到的抗血清效价为1/2048(微量沉淀反应).Differential centrifugation and sucrose cushion centrifugation were used to comparethree methods for purification of soybean mosaic virus.The yields were 24.5,47.1,and199.2mg per kg of the infected leaves.The purified virus was infective.Antisera with a titreof 1/2048(microprecipitation test)were prepared by injecting the purified virus intorabits.

关 键 词：大豆花叶病毒 提纯 

分 类 号：S[农业科学]