高铜日粮对肉鸡肝抗氧化功能和肝细胞凋亡的影响  

Effect of High-copper Diets on Liver Antioxidant Function and Liver Cell Apoptosis of Broilers

作　　者：任常宝[1] 胡锴[1] 颜诚[1] 苏荣胜[1] 郭剑英[1] 潘家强[1] 唐兆新[1,2] 

机构地区：[1]华南农业大学兽医学院,广东广州510642 [2]广东省兽用生物制品技术研究与应用企业重点实验室,广东新兴527400

出　　处：《中国畜牧兽医》2011年第11期18-22,共5页China Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金资助项目(30871900)

摘　　要：选用200只1日龄健康商品代AA肉鸡,随机分成4组,使用硫酸铜作为铜源,饲喂玉米―豆粕型基础日粮,对照组饲料铜含量为11mg/kg,3个高铜试验组饲料铜含量分别为110、330和550mg/kg,试验至50日龄结束,探讨高铜日粮对肉鸡肝抗氧化功能和肝细胞凋亡的影响。结果显示,3个高铜试验组超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)活性先呈代偿性升高,后表现降低,丙二醛(MDA)表现为代偿性升高。随着饲养周期的延长和日粮铜含量的增加,高铜组肉鸡肝细胞凋亡率升高并逐渐出现比较明显的DNA断裂条带。结果表明,日粮铜含量为330mg/kg及其以上可引起肝脏抗氧化功能受损,肝细胞凋亡率升高。200 one-day-old commercial generation AA broilers were randomly divided into four groups.In addition to the control group（Cu 11 mg/kg）,three experimental groups were set up,they were groups of 110,330 and 550 mg/kg respectively.All of the experimental broilers were fed with corn-soybean meal for 50 days,and effect of high-copper diets on liver antioxidant function and liver cell apoptosis of broilers were assayed.The results showed that 3 high copper group of SOD and GSH-Px increased compensatorily,consequently,a performance of decline;and MDA compensatory increased.With time prolonging and the content of copper increasing,liver cell apoptosis rate rise and DNA fracture strip obviously appeared.It showed that daily ration of copper content 330 mg/kg or more could cause liver antioxidant function was impaired,liver cell apoptosis rate increased.

关 键 词：高铜 肉鸡 肝脏 抗氧化 细胞凋亡 

分 类 号：S816[农业科学—饲料科学]