马兜铃酸Ⅰ诱导肾小管上皮细胞凋亡的检测  

Investigate the Effect of Aristolochic Acid Ⅰ in Inducing Apoptosis of the RTECs

作　　者：彭晓兰[1] 董晓凯[1] 廖芳芳[1] 高磊[1] 张中文[1] 吴国娟[1] 

机构地区：[1]北京农学院动物科学技术学院,北京102206

出　　处：《中国畜牧兽医》2011年第11期61-65,共5页China Animal Husbandry & Veterinary Medicine

基　　金：北京市自然科学基金项目(5102014;6092004);国家自然科学基金(30972215);北京市属高校人才强教计划资助项目(PHR2009-07135);北京市教委(KM201010020008)

摘　　要：本试验旨在培养小鼠肾小管上皮细胞(renal tubular epithelial cells,RTECs),探讨马兜铃酸Ⅰ(aristolochic acidⅠ,AAⅠ)诱导小鼠肾小管上皮细胞的凋亡情况。AAⅠ与RTECs反应后,从细胞形态学、DNA Ladder、流式细胞仪3个方面检测AAⅠ诱导RTECs的凋亡情况。AAⅠ作用后,随着浓度的增高,上皮细胞失去上皮细胞表型,在20～80μg/mL作用24h后,细胞都出现晚期凋亡的特征;在20μg/mL AAⅠ的作用下早期凋亡的比率随作用时间的延长而增高。AAⅠ可诱导小鼠原代RTECs产生凋亡,凋亡随着作用时间的延长和药物浓度的增大而加剧。To investigate the effect of AAⅠ in inducing apoptosis of the renal tubular epithelial cells（RTECs） in mouse,mouse primary RTECs were cultured and identificated by morphology observation.After RTECs treated with or without AAⅠ,DNA ladder,flow cytometry analysis（FCAS） the apoptosis of the RTECs.Mouse RTECs could be successfully cultured,with the increase of AAⅠ,cells lost epithelial characteristics.After 24 h,20 to 80 μg/mL of AAⅠ stimulated RTECs,cells apparence late apoptosis.The apoptosis of mouse origin RTECs could be induced by AAⅠ,the degree of apoptosis is along with the increasing of AAⅠ action time and density.

关 键 词：马兜铃酸Ⅰ 肾小管上皮细胞 细胞凋亡 

分 类 号：S852.33[农业科学—基础兽医学]