转人乳铁蛋白基因奶牛多重PCR检测方法的研究  

Multiplex PCR Detection of Exogenous Gene in hLF Transgenic Cow

作　　者：朱振营[1] 李风翠[1,2] 林祥梅[1] 吴绍强[1] 仇松寅[1] 李宁[3] 王建武[3] 薛振华[4] 刘建[1] 张书霞[2] 

机构地区：[1]中国检验检疫科学研究院动植物检疫研究所,北京100029 [2]南京农业大学,南京210095 [3]中国农业大学农业生物技术国家重点实验室,北京100193 [4]北京市畜牧兽医总站,北京100107

出　　处：《中国畜牧兽医》2011年第11期73-76,共4页China Animal Husbandry & Veterinary Medicine

基　　金：转基因重大专项课题转基因产品检测新技术研究(2009ZX08012-001B)

摘　　要：本研究旨在建立转基因奶牛多重PCR快速检测方法,为转基因动物及产品出入境检测技术平台的建立提供技术支持,并为转基因动物及产品检测技术标准的制定提供参考。根据牛物种特异性基因(mtDNA)设计奶牛内源基因引物,根据外源基因人乳铁蛋白基因(human lactoferrin,hLF)和新霉素磷酸转移酶基因(NPTⅡ)设计特异性引物,优化反应条件,建立转基因奶牛多重PCR检测方法。本方法敏感、快速、特异,一个反应可以检测多个基因片段,可有效用于转基因奶牛外源基因的检测。For developing the typical testing standard of transgenic mammals and its derived food and the technical support for entry﹠exit animal quarantine,detection of the transgenic component in transgenic cow by multiplex PCR was developed in this study.The endogenous mtDNA gene specific for cow identification,exotic hLF gene,marker NPTⅡ gene were amplified according to different primers.The process of the multiplex PCR reaction was optimized.The system described herein represent simple,accurate,and sensitive detection methods in which only one reaction was necessary to detect multiple target sequences that can be reliably used for the identification of specific lines of transgenic cows.

关 键 词：转基因奶牛 多重PCR hLF基因 

分 类 号：Q78[生物学—分子生物学]