大鼠骶髓前角的胆碱能神经元与P物质样末梢的突触联系 双重免疫组织化学染色法研究  

THE SYNAPSES BETWEEN CHOLINERGIC NEURONS AND SUBSTANCE P TERMINALS OF THE RAT SACRAL VENTRAL HORN A DOUBLE IMMUNOSTAINING METHOD

作　　者：彭裕文[1] 蒋文华[1] 

机构地区：[1]上海医科大学解剖学教研室,上海200032

出　　处：《解剖学报》1990年第S1期1-5,129,共6页Acta Anatomica Sinica

基　　金：国家自然科学基金资助

摘　　要：用双重免疫组织化学染色法,在光镜和电镜下观察了大鼠骶髓前角的胆碱能神经元与SP样末梢间的关系。胆碱能神经元被抗胆碱乙酰转移酶(ChAT)的单克隆抗体标记,用抗生物素—生物素技术,以β-半乳糖苷酶作为标记物,被吲哚-β-半乳糖苷(IbGa)反应产物染成蓝绿色。在同一切片上,SP样末梢被抗SP的多克隆抗体标记,用PAP技术,被DAB反应产物染成棕色。在光镜下,染成蓝绿色的ChAT样神经元和染成棕色的SP样纤维在前角出现。在电镜下,前角的ChAT样树突与SP样末梢之间,存在着许多不对称性的轴树突触(Gray Ⅰ型)、而几乎没有轴体突触和对称性的突触(Gray Ⅱ型)。这些结果显示骶髓前角的SP样终末与ChAT样运动神经元形成直接的突触,并提示这些突触主要是兴奋性的,对控制肌肉收缩有重要意义。The relationships between cholinergic neurons and SP terminals were examined in the rat sacral ventral horn at the light and electron microscopic levels by means of double immunostaining methods. Cholinergic neurons were labeled by a monoclonal antibody to choline acetyltransferase (CHAT) with the avidin-biotin technique and stained bluish-green by indolyl-β-galactoside reaction products with β-galactosidase as a marker. On the same sections, SP fibers were labeled by polyclonal antisera to SP after application of the peroxidase-antiperoxidase (PAP) method and stained brown by diaminobenzidine (DAB)reaction. At the light microscopic level CHAT-I neurons stained bluish-green and SP- I fibers Stained brown were found in the ventral horn. At the electron microscopic level, many asymmetrical axodendritic synapses(type I of Gray)were observed between CHAT-I dendrites and SP-I terminals in the ventral horn, but axosomatic synapses and symmetrical synapses (type I of Gray) were hardly detected. These results indicate that SP-I terminals make direct synapses with CHAT-I motoneurons of sacral ventral horn. These synapses may be predominantly excitatory and have importance in the control of muscular constriction.

关 键 词：骶髓前角 胆碱能神经元 SP 样末梢突触 双重免疫染色法 

分 类 号：R322[医药卫生—人体解剖和组织胚胎学]