叶酸对酒精所致肝脏损害保护作用的实验研究  

EXPERIMENTAL STUDY OF THE PROTECTIVE EFFECT OF FOLIC ACID ON LIVER DAMAGE INDUCED BY ALCOHOL

作　　者：熊希凯[1] 陈艳贤[1] 董大翠[1] 张艳[1] 

机构地区：[1]同济医科大学解剖学教研室,武汉430030

出　　处：《解剖学报》1990年第S1期17-22,132,共7页Acta Anatomica Sinica

摘　　要：酒精对肝脏有损害作用是众所周知的。本文重点研究叶酸对酒精所致的肝损害是否有保护作用。实验用68只NIH小鼠并分成三组,正常组20只(A组),酒精组24只(B组)、叶酸酒精组24只(C组)分别用上述物质灌喂1、2、3、4周后,分期处死动物。用组织化学、电镜等方法观察。结果表明:叶酸可使肝脏SDH、Mg^(++)—ATPase、G-6-Pase的活性明显增强,并使糖原合成增加;使ACP活性降低。对线粒体、内质网等超微结构无损害。这提示叶酸对已受酒精损害的肝细胞的功能和结构有明显的保护作用。68 NIH Mice (24-25g) were divided into three groups. Group A was normal control, group B was treated with 54% alcohol in the beverages 0.25ml/per day, and group C was administrated 54% alcohol mixed with folic acid 0.25ml/per day. The histochemical reaction and ultrastructural changes in liver were observed. The reaction of glycogen (PAS reaction), Mg-ATPase SDH LDH G-6-Pasc and ACP was demonstrated respectively by Chayen's, Wachstein-Meisel, Lojda, Bancroft's method, and some procedures of these techniques were modified. The results showed that under the protection effect of acid folic, the regularities seemed to be present in the changes of the following histochemical reactions in the liver cells of the group C (treaded by acid folic) the PAS reaction and the activities of Mg-ATPase SDH LDH G-6-Pase were similar to the group A and apparently higher than that in group B. The activities of ACP were lower those in group B(P<0.01).Ultrastructural changes: comparing the damages of mitochondria and endoplasmic reticulum. The group B was conspicuous whereas the group C appeared not. As mentioned above it suggested that the folic acid has protective effect on the function and structure of liver cells.

关 键 词：叶酸 酒精 肝 组织化学 超微结构 

分 类 号：R322[医药卫生—人体解剖和组织胚胎学]