幼龄母羊超数排卵及卵母细胞成熟效果分析  

Study on Follicular Development of Female Lamb and Technology of Oocyte Maturation

作　　者：张兴会[1] 张世伟[2] 宋先忱[1] 郭丹[2] 豆兴堂[1] 薛冰[2] 常青[3] 

机构地区：[1]辽宁省辽宁绒山羊原种场有限公司,辽宁盖州115200 [2]辽宁省畜牧科学研究院,辽宁辽阳111000 [3]吉林农业大学,吉林长春130118

出　　处：《中国畜牧兽医》2011年第11期136-139,共4页China Animal Husbandry & Veterinary Medicine

基　　金：国家科技支撑计划"常年长绒型辽宁绒山羊新品系快速扩繁技术集成与应用"(2009BADA5B02);国家绒毛用羊产业技术体系项目(nycytx-40-16nycytx-40-26)

摘　　要：本试验以辽宁绒山羊卵巢为研究对象,研究不同激素处理对羔羊卵泡发育的影响,比较了羔羊超排处理后与普通成年母羊卵巢内的卵母细胞体外成熟过程的差异,同时还对比了不同配方的培养液对羔羊卵母细胞体外成熟率的影响。结果表明,辽宁绒山羊母羔早期利用采卵的最佳时期为5～6周龄,体重在5.6～7.5kg之间;母羔最佳的超数排卵方案是依次注射FSH 40mg 3次、PMSG 400IU 1次,间隔时间12h;在成熟培养液中添加12mg/mL的BSA可以替代血清培养液(10%FBS),不影响卵母细胞最终成熟;母羔卵母细胞体外成熟率显著低于成年母羊。This research was conducted to determine the effect of different hormones or treatments methods on lamb＇s follicular oocytes development,and the difference of oocytes＇ maturation process in vitro between superovulated lamb and normal adult ewe was studied.Besides,the maturing rate of lamb＇s oocytes in vitro based on different prescription of culture solution was determined.The results suggested as following： the optimal age for early using of Liaoning Cashmere goats＇ lamb was 5 to 6 weeks,with the body weight between 5.6 to 7.5 kg,the optimal method of lamb＇s superovulation was injecting FSH 40 mg three times and PMSG 400 IU once in sequence with the interval of 12 hours,adding 10% FBS（fetal bovine serum） into culture solution can be replaced by 12 mg/mL BSA,which influenced nothing to the maturation of lamb＇s oocytes.However,the matured rate of oocytes in vitro of superovulated lamb＇s was significantly lower than normal adult ewe＇s.

关 键 词：幼龄母羊 超数排卵 卵母细胞 体外成熟 

分 类 号：S814.4[农业科学—畜牧学]