胚胎分割技术在肉牛繁育中的研究与应用  

Study and Application on Beef Cattle Embryo Bisection

作　　者：裴燕 冯春涛 周艳华[2,3] 朱士恩[2,3] 余文莉 

机构地区：[1]石家庄天泉良种奶牛有限公司,河北石家庄050051 [2]国家肉牛产业技术体系遗传育种与繁殖功能研究室,北京100193 [3]中国农业大学动物科技学院,北京100193

出　　处：《中国畜牧兽医》2011年第11期147-150,共4页China Animal Husbandry & Veterinary Medicine

基　　金：现代农业产业技术体系建设专项;"十二五"科技支撑课题(2011BAD19B02);河北省科技支撑项目(11220106D)

摘　　要：本试验采用弗莱维赫肉牛和日本和牛作为供体,本地黄牛为受体,进行同期发情和超数排卵,共得到可用胚胎234枚,分为4个处理:新鲜全胚113枚、新鲜双半胚55枚、冷冻全胚56枚和冷冻双半胚10枚,进行移植。结果表明,新鲜双半胚的妊娠率为65.45%,略高于新鲜全胚移植的妊娠率(63.72%),差异不显著(P>0.05)。冷冻双半胚产犊率为20.00%,低于冷冻全胚移植的妊娠率(39.29%),差异不显著(P>0.05),而冷冻组的妊娠率均显著低于新鲜组(P<0.05)。新鲜双半胚的产犊率为95.56%,双犊率为35.55%,犊牛存活率为72.09%。新鲜全胚组的产犊率为63.16%,显著低于新鲜双半胚组(P<0.05),但犊牛存活率却显著高于新鲜半胚组(90.00%和72.09%)(P<0.05)。此外,全胚与双半胚移植后产生的犊牛初生重之间并无显著差异(P>0.05)。Fleckvieh beef cattle and Japanese Black beef were used as donor,local cattle as receptors in this study,then estrus synchronization and superovulation were conducted,and 234 embryos were obtained,of which were divided into four groups,including 113 fresh whole embryos,55 fresh double half embryos,56 frozen whole embryos and 10 frozen double half embryos.The transplantation effect of whole embryo and double half embryo were detected.The results indicated that the pregnancy rate of fresh double half embryo was 65.45%,which was higher than fresh whole embryo group（63.72%）,but no significant difference（P〉0.05）.Frozen double half embryo group was 20.00%,which was not significantly lower than the corresponding whole embryo group（39.29%）（P〉0.05）.However,both frozon groups were significantly lower than fresh groups.The calving rate of fresh double half embryo was 95.56%,of which 35.55% were twins and 72.09% were survived.The calving rate of fresh whole group was 63.16%,which was significantly lower than the fresh double half group（P〈0.05）,but the surviving rate was significantly higher than the fresh double half group（90.00% and 72.09%）（P〈0.05）.As for the birth weight,there was no significant difference among all of the four groups.

关 键 词：胚胎分割 双半胚 移植妊娠率 产犊率 初生重 

分 类 号：S814.6[农业科学—畜牧学]