鸭瘟强毒株和疫苗株对雏鸭IFN-γ基因表达的影响  

Dynamics of IFN-γ mRNA Expression in Ducks Induced by Duck Plague Virus and Attenuated Virus

作　　者：谢秀兰[1] 杨发龙[2] 李阳友[3] 马小明[1] 岳华[2] 

机构地区：[1]宁夏农林科学院草畜工程中心,宁夏银川750002 [2]西南民族大学生命科学与技术学院,四川成都610041 [3]川北医学院实验动物中心,四川南充637002

出　　处：《中国畜牧兽医》2011年第11期174-177,共4页China Animal Husbandry & Veterinary Medicine

基　　金："十一五"国家科技支撑计划重大项目(2006BAD06A11)

摘　　要：本试验旨在探讨不同毒力的鸭瘟病毒(DPV)感染对雏鸭INF-γmRNA表达水平的影响,为DPV的感染与免疫机制提供理论依据。应用实时荧光定量PCR技术,对接种了鸭瘟强毒株和疫苗株的雏鸭肝脏及外周血淋巴细胞(PBL)中IFN-γmRNA的表达水平及鸭瘟病毒(DPV)的荷载量进行动态定量监测。结果表明:①感染鸭瘟强毒后,IFN-γmRNA在肝脏中的表达没有明显规律,PBL中IFN-γmRNA表达水平很高,在此期间(1～12h),病毒DNA量很少。IFN-γmRNA表达量在6h后大幅下降,直到144h都非常低,而病毒的载量逐渐增大,至144h时达到峰值。②接种弱毒疫苗后,肝脏中IFN-γmRNA表达水平较高且稳定,至12h达到顶峰,约比对照高出8倍。PBL中IFN-γmRNA表达量较低且不稳定。弱毒DNA荷载量稳定上升,但载量约比强毒低两个数量级。病毒DNA在PBL检测不到。IFN-γ在抵抗鸭瘟强毒中发挥了重要作用;IFN-γ在肝脏中高水平的表达可能是鸭瘟疫苗的免疫机制之一。To understand the mechanisms of DPV infection and immunity,the IFN-γ mRNA expression was investigated after infection with virulent and attenuated duck plague virus.The mRNA levels of IFN-γ in liver and PBL were evaluated dynamically after inoculation with virulent and attenuated DPV respectively by real-time PCR.The virus loads were also assessed.Results：①After infection by virulent DPV,IFN-γ mRNA levels in liver didn＇t show regulatory changes while high level of IFN-γ mRNA levels in PBL and few virus DNA could be detected in the early stage of infection（1-12 h）.IFN-γ mRNA expression decreased dramatically after 6 h post infection and persisted in a very low level until death（144 h）.Virus load increased gradually and reached peak at 144 h.②After vaccination with attenuated DPV strain,there were persist and high levels of IFN-γ mRNA expression in liver,reached peak at 12 h（8 times higher than control）,while the level in PBL was lower and unstable.The attenuated DPV loads increased dramatically with a relative low copy number in liver.However,the virus DNA could not be detected in PBL.IFN-γ possibly plays an important role in protecting ducks against virulent virus infection.It is also suggested that high level expression of IFN-γ in liver induced by attenuated DPV could enhance immunity against DPV infection.

关 键 词：IFN-Γ 鸭瘟病毒 强毒 弱毒 

分 类 号：S852.47[农业科学—基础兽医学]