鸡骨草药材指纹图谱研究  

Research on HPLC Fingerprint of Abrus Cantoniensis

作　　者：黄勇斌[1] 孙毅东[1] 李耿[2] 梁幼雅[2] 周秀[2] 侯力儒[2] 

机构地区：[1]广东省中医院珠海医院,广东珠海519015 [2]广州中医药大学中药学院,广东广州510006

出　　处：《今日药学》2011年第5期280-282,301,共4页Pharmacy Today

摘　　要：目的建立鸡骨草药材的指纹图谱。方法采用RP-HPLC高效液相色谱法,Phenomenex-ODS色谱柱(250 mm×4.0 mm,5μm),甲醇-0.5%冰醋酸溶液,梯度洗脱,流速1.0 ml/min,检测波长270 nm。结果建立了鸡骨草药材指纹图谱共有模式,显示5个共有峰,指认了相思子碱1个特征峰。结论 HPLC指纹图谱中各峰分离度较好,方法具有较好的精密度、重现性和稳定性,符合指纹图谱的要求,可用于鸡骨草药材质量控制。Objective To establish a HPLC fingerprint analysis method for Abrus cantoniensis.Methods Phenomenex-ODS column（250 mm×4.0 mm,5 μm） was used,with gradient elution by the mobile phase consisted of Methanol-0.5% glacial acetic acid.The flow rate was 1.0 ml/min,and the detection wavelength was 270 nm.Results A mutual model of finger-print chromatogram was established.5 common peaks were displayed.A characteristic peak of abrine was identified.Conclusion The characteristic peaks are selected with good resolution,and the method is reproducible,accurate,and stable.It can be used for the quality control of Abrus cantoniensis.

关 键 词：指纹图谱 鸡骨草 相思子碱 高效液相色谱法 

分 类 号：R284[医药卫生—中药学]