正交试验法优选前列宁胶囊的壳聚糖纯化工艺  

Optimization of the Chitosan Purification Process of Qianliening Capsuleby Orthogonal Test

作　　者：徐伟[1] 李煌[1] 林久茂[2] 周建衡[3] 陈瑞龙 郑海音[3] 洪振丰[3] 

机构地区：[1]福建中医药大学药学院,福州350108 [2]福建中医药大学中西医结合研究院 [3]福建中医药大学中西医结合学院 [4]台湾嘉南药理科技大学

出　　处：《浙江中医药大学学报》2011年第4期571-574,共4页Journal of Zhejiang Chinese Medical University

基　　金：国家自然科学基金(No:81072927);福建省科技厅重点项目(No:2009I0011);福建省中西医结合老年性疾病重点实验室开放课题基金资助项目;2008年陈可冀中西医结合发展基金(No:2008J1004-17CKJ2008054)~~

摘　　要：[目的]筛选前列宁胶囊的最佳壳聚糖纯化工艺。[方法]采用天然絮凝剂壳聚糖纯化前列宁胶囊,运用正交设计,以浸膏得率、多糖保留率、大黄素为指标,考察壳聚糖浓度、反应温度、pH值和静置时间等影响因素,综合评价,筛选其最佳纯化工艺。[结果]最佳纯化工艺参数为:壳聚糖浓度20%,反应温度60℃,反应液pH=6,静置时间24h,提取液的出膏率大大降低。[结论]采用壳聚糖澄清剂纯化前列宁胶囊除杂效果好,制剂质量稳定。[Objective]To screen the best purification technology of Qianliening capsule.[Method]The optimization purification technology of Qianliening capsule was investigated,which uses orthogonal design with the dry extract rate,the polysaccharide contain rate and emodin as index,to justify the influence of chitosan concentration,reaction temperature,PH and standing time.[Result]The optimal condition for the chitosan purification technology of Qianliening capsule was chitosan concentration 20%,reaction temperature 60 degrees,the reaction liquid pH7.0 and standing time 24h.According to the technological parameters of purification,the yield of extract is reduced greatly.[Conclusion]The chitosan clarification can be used for purifying Qianliening capsule.The quality of preparation is stable.

关 键 词：正交试验 前列宁胶囊 壳聚糖 纯化工艺 

分 类 号：R282.71[医药卫生—中药学]