正交设计法优选土茯苓中落新妇苷及总黄酮的提取工艺  

Optimal Extraction of Total Flavones and Astilbin in Smilax Glabra Roxb by Orthogonal Design

作　　者：林耿丰[1] 梁伟杰[1] 肖凤霞[2] 邓少东[2] 邓超明[2] 黎杏仪[2] 

机构地区：[1]广东省中医院,广东广州510120 [2]广州中医药大学中药学院,广东广州510006

出　　处：《亚太传统医药》2011年第6期39-42,共4页Asia-Pacific Traditional Medicine

摘　　要：目的:研究超声法提取土茯苓中落新妇苷及总黄酮的最佳工艺条件。方法:采用正交试验法研究落新妇苷和总黄酮的提取工艺,其中落新妇苷含量用HPLC法测定,总黄酮含量用紫外分光光度法测定。结果:土茯苓中落新妇苷最佳提取工艺为加入甲醇30mL,超声提取15min,超声3次。总黄酮的最佳提取工艺为料液比1∶50,加入50%乙醇,超声提取60min。结论:所建立的方法简便、准确、重复性好,可用于土茯苓药材中落新妇苷及总黄酮的含量测定及其质量控制。Objective：To optimize and establish the extraction of total flavones and astilbin in Smilax Glabra Roxb.Methods：The orthogonal design was used in the research on the ultrasonic-assisted technique extraction technique,the content of total flavones and astilbin was determined by UV and HPLC methods.Results：The optimal extraction condition of astilbin was extracting with 30 mL methanol with 15min for 3 times,and the total flavones was extracting with 50 times volume of sample weight with 50% alcohol for 1h.Conclusion：The method is simple,rapid,accurate and applicable to the extraction process of total flavones and astilbin in Smilax Glabra Roxb.

关 键 词：土茯苓 落新妇苷 总黄酮 正交试验 

分 类 号：R284.2[医药卫生—中药学]