急性高糖环境对大鼠骨形成的影响  

Influence of acute high level glucose on rat bone formation

作　　者：邓艳华[1] 赵琳[1] 刘冬梅[1] 张宏利[1] 刘建民[1] 

机构地区：[1]上海交通大学医学院附属瑞金医院内分泌代谢科上海市内分泌代谢病研究所上海市内分泌代谢病临床医学中心,上海200025

出　　处：《诊断学理论与实践》2011年第5期444-448,共5页Journal of Diagnostics Concepts & Practice

摘　　要：目的:观察急性高糖环境对大鼠骨形成的影响。方法:将原代新生SD大鼠成骨细胞分为对照组(葡萄糖浓度为5.5 mmol/L)和高糖组(葡萄糖浓度为22.0 mmol/L),高糖组分别于作用后24、48、72 h收集细胞。向成年SD大鼠颈内静脉持续灌注生理盐水(对照组)和50%葡萄糖(高糖组),持续灌注24、48、72 h后处死,并取胫腓骨骨组织。分别提取成骨细胞和胫腓骨骨组织中骨钙素(OC)、Ⅰ型胶原(COL1)、Runt相关转录因子2(RUNX2)、碱性磷酸酶(ALP)、c-JUN和胰岛素样生长因子1(IGF-1)的mRNA及相应蛋白,并用实时荧光定量PCR和蛋白印迹法检测骨形成相关基因的表达。结果:急性高糖环境下,相较对照组,大鼠成骨细胞ALP、COL1、RUNX2、IGF-1、OC的mRNA表达在高糖培养48 h升高(P<0.05);成骨细胞经高糖处理后,P38MAPK磷酸化水平逐渐增加,在高糖培养48 h时磷酸化水平达最高。在不同高糖处理时间组SD大鼠胫腓骨骨组织中,OC、ALP、COL1、RUNX2、IGF-1、c-JUN的mRNA表达有差异,P38MAPK磷酸化水平在高糖培养48 h达最高。结论:骨形成过程中,成骨细胞可能在急性高糖环境下出现增殖分化增加,并通过MAPK通路进行调控。Objective To investigate the influence of acute high level glucose on rat bone formation in vivo and vitro.Methods Primary rat osteoblasts were incubated in medium containing control level glucose（5.5 mmol/L） and high level glucose（22.0 mmol/L） and were collected 24-,48-and 72-hour afterwards.Also,SD rats were infused with either glucose（50% glucose） or normal saline（control） for 24,48 and 72 hours and then sacrificed,and bone tissues of tibia and fibula were collected.Expressions of OC,COL1,RUNX2,ALP,c-JUN,IGF-1 mRNA and protein in primary osteoblasts and tissues of tibia and fibula were tested by real-time fluorescence PCR and Western blot.Results With the environment of acute high level glucose,expressions of ALP,COL1,RUNX2,IGF-1,OC,c-JUN mRNA were much higher in 48-hour treated group.Conclusions Proliferation and differentiation of osteoblasts in the process of bone formation may increase under the influence of acute high level glucose.The effect is modulated through MAPK signaling pathway.

关 键 词：急性高糖 骨形成 增殖 分化 

分 类 号：R587.1[医药卫生—内分泌]