Down-regulation of STAT3 expression by vector-based small interfering RNA inhibits pancreatic cancer growth  

作　　者：Chen Huang Guang Yang Tao Jiang Jun Cao Ke-Jian Huang Zheng-Jun Qiu 

机构地区：[1]Department of General Surgery, Affiliated First Peo-ple＇s Hospital, Shanghai Jiao Tong University, Shanghai 200080,China [2]Department of General Surgery, Taian CentralHospital, Taian 271000, Shandong Province, China [3]Shanghai Key Laboratory ofPancreas Disease, Shanghai 200080, China [4]Pancreatic Cancer Center ofShanghai Jiaotong University, Shanghai 200080, China

出　　处：《World Journal of Gastroenterology》2011年第25期2992-3001,共10页世界胃肠病学杂志（英文版）

基　　金：Supported by A Grant from the Science and Technology Commission of Shanghai Municipality,No. 09QA1404600;a grant from the Affiliated First People’s Hospital of ShanghaiJiao Tong University,No. 0801

摘　　要：AIM:To evaluate the effect of RNA interference (RNAi) mediated silence of signal transduction and activation of transcription (STAT)3 on the growth of human pancreatic cancer cells both in vitro and in vivo.METHODS:STAT3 specific shRNA was used to silence the expression of STAT3 in pancreatic cancer cell line SW1990.The anti-growth effects of RNAi against STAT3 were studied in vitro and in experimental cancer xenografts in nude mice.The potential pathways involved in STAT3 signaling were detected using reverse transcription polymerase chain reaction and western blotting.RESULTS:The expression of the STAT3 was inhibited using RNAi in SW1990 cells.RNAi against STAT3 inhibited cell proliferation,induced cell apoptosis and significantly reduced the levels of CyclinD1 and Bcl-xL when compared with parental and control vector-transfected cells.In vivo experiments showed that RNAi against STAT3 inhibited the tumorigenicity of SW1990 cells and significantly suppressed tumor growth when it was directly injected into tumors.CONCLUSION:STAT3 signaling pathway plays an important role in the progression of pancreatic cancer,and silence of STAT3 gene using RNAi technique may be a novel therapeutic option for treatment of pancreatic cancer.AIM： To evaluate the effect of RNA interference （RNAi） mediated silence of signal transduction and activation of transcription （STAT）3 on the growth of human pan- creatic cancer cells both in vitro and in vivo. METHODS： STAT3 specific shRNA was used to silence the expression of STAT3 in pancreatic cancer cell line SW1990. The anti-growth effects of RNAi against STAT3 were studied in vitro and in experimental cancer xenografts in nude mice. The potential pathways involved in STAT3 signaling were detected using reverse transcrip- tion polymerase chain reaction and western blotting.RESULTS： The expression of the STAT3 was inhibited using RNAi in SW1990 cells. RNAi against STAT3 inhib- ited cell proliferation, induced cell apoptosis and signif- icantly reduced the levels of CyclinD1 and Bcl-xl_ when compared with parental and control vector-transfected cells. In vivo experiments showed that RNAi against STAT3 inhibited the tumorigenicity of SW1990 cells and significantly suppressed tumor growth when it was directly injected into tumors.CONCLUSION： STAT3 signaling pathway plays an important role in the progression of pancreatic cancer, and si- lence ofSTAT3 gene using RNAi technique may be a novel therapeutic option for treatment of pancreatic cancer.

关 键 词：Signal transduction and activation of tran-scription 3 RNA interference Pancreatic cancer GROWTH 

分 类 号：Q756[生物学—分子生物学] Q522