P16 gene hypermethylation and hepatocellular carcinoma:A systematic review and meta-analysis  

作　　者：Jia-Jie Zang Feng Xie Jin-Fang Xu Ying-Yi Qin Rong-Xi Shen Jia-Mei Yang Jia He 

机构地区：[1]Department of Health Statistics, Second Military Medical Univer-sity, Shanghai 200433, China [2]Department of Special Treatment and Liver Transplantation, Eastern HepatobiliarySurgery Hospital, Second Military Medical University, Shanghai200438, China

出　　处：《World Journal of Gastroenterology》2011年第25期3043-3048,共6页世界胃肠病学杂志（英文版）

基　　金：Supported by The Ministry of Science and Technology of China,No. 2009ZX09312-025 and No. 2008ZX10002-018

摘　　要：AIM:To quantitatively investigate the effect of p16 hypermethylation on hepatocellular carcinoma (HCC) and hepatocirrhosis using a meta-analysis of available casecontrol studies.METHODS:Previous studies have primarily evaluated the incidence of p16 hypermethylation in HCC and corresponding control groups,and compared the incidence of p16 hypermethylation in tumor tissues,pericancer liver tissues,normal liver tissues and non-tumor liver tissues with that in other diseases.Data regarding publication information,study characteristics,and incidence of p16 hypermethylation in both groups were collected from these studies and summarized.RESULTS:Fifteen studies,including 744 cases of HCC and 645 non-tumor cases,were identified for meta-analysis.Statistically significant odds ratios (ORs) of p16 hypermethylation were obtained from tumor tissues and non-tumorous liver tissues of HCC patients (OR 7.04,95% CI:3.87%-12.78%,P < 0.0001),tumor tissues of HCC patients and healthy liver tissues of patients with other diseases (OR 12.17,95% CI:6.64%-22.31%,P < 0.0001),tumor tissues of HCC patients and liver tissues of patients with non-tumorous liver diseases (OR 6.82,95% CI:4.31%-10.79%,P < 0.0001),and cirrhotic liver tissues and non-cirrhotic liver tissues (OR 4.96,95% CI:1.45%-16.96%,P=0.01).The pooled analysis showed significantly increased ORs of p16 hypermethylation (OR 6.98,95% CI:4.64%-10.49%,P < 0.001) from HCC tissues and cirrhotic tissues.CONCLUSION:P16 hypermethylation induces the inactivation of p16 gene,plays an important role in hepatocarcinogenesis,and is associated with an increased risk of HCC and liver cirrhosis.AIM： To quantitatively investigate the effect of p16 hypermethylation on hepatocellular carcinoma （HCC） and hepatocirrhosis using a meta-analysis of available casecontrol studies. METHODS： Previous studies have primarily evaluated the incidence of p16 hypermethylation in HCC and corresponding control groups, and compared the incidence of p16 hypermethylation in tumor tissues, pericancer liver tissues, normal liver tissues and non-tumor liver tissues with that in other diseases. Data regarding publication information, study characteristics, and incidence of p16 hypermethylation in both groups were collected from these studies and summarized. RESULTS： Fifteen studies, including 744 cases of HCC and 645 non-tumor cases, were identified for metaanalysis. Statistically significant odds ratios （ORs） of p16 hypermethylation were obtained from tumor tis- sues and non-tumorous liver tissues of HCC patients （OR 7.04, 95% CI： 3.87%-12.78%, P 〈 0.0001）, tu- mor tissues of HCC patients and healthy liver tissues of patients with other diseases （OR 12.17, 95% CI： 6.64%-22.31%, P 〈 0.0001）, tumor tissues of HCC pa- tients and liver tissues of patients with non-tumorous liver diseases （OR 6.82, 95% CI： 4.31%-10.79%, P 〈 0.0001）, and cirrhotic liver tissues and non-cirrhotic liver tissues （OR 4.96, 95% CI： 1.45%-16.96%, P = 0.01）. The pooled analysis showed significantly in- creased ORs of p16 hypermethylation （OR 6.98, 95% CI： 4.64%-10.49%, P 〈 0.001） from HCC tissues and cirrhotic tissues. CONCLUSION： P16 hypermethylation induces the inactivation of p16 gene, plays an important role in hepatocarcinogenesis, and is associated with an increased risk of HCC and liver cirrhosis.

关 键 词：P16 hypermethylation Hepatocellular carci-noma Liver cirrhosis META-ANALYSIS Odds ratio 

分 类 号：Q523.8[生物学—生物化学] Q343