兔胎盘来源间充质干细胞修复兔桡骨骨缺损的实验研究  

Osteogenic capacity of placenta-derived mesenchymal stem cells in rabbits

作　　者：卢遥[1] 王静成[1] 顾加祥[1] 颜连启[1] 冯新民[1] 戴善和[1] 王强[1] 虞海流[1] 解慧琪[2] 李秀群[2] 杨志明[2] 

机构地区：[1]苏北人民医院骨科,江苏省扬州市225001 [2]四川大学华西医院生物治疗国家重点实验室、干细胞与组织工程研究室

出　　处：《中华创伤骨科杂志》2011年第11期1060-1065,共6页Chinese Journal of Orthopaedic Trauma

基　　金：国家自然科学基金（30670734）;四川省科技厅青年基金（04ZQ026-038）

摘　　要：目的探讨兔胎盘来源间充质干细胞（PMSCs）构建的组织工程化骨修复兔桡骨节段性骨缺损的能力。方法取24只大耳白兔，于兔双侧桡骨中下段制造1．5cm的节段性骨缺损，左侧骨缺损用PMSCs构建的组织工程化骨桥接（实验组），右侧用骨髓基质干细胞（BMSCs）构建的组织工程化骨桥接（对照组）。实验动物双侧于术后2、4、8、12周摄X线片和取材，对双侧X线片进行影像学评分；对取材行大体、组织学检查、生物力学性能测定和扫描电镜观察。结果术后2、4、8、12周实验组与对照组X线评分平均分别为（3．72±0．24）和（3．49±0．29）分、（6．24±0．41）和（5．91±0．45）分、（8．15±0．67）和（8．87±0．59）分、（11．39±0．86）和（12．04±0．93）分，两组比较差异均无统计学意义（P〉0．05）。组织学观察显示膜内化骨和软骨内化骨均参与了双侧骨缺损愈合的成骨过程，但以软骨内化骨为主。术后2、4、8、12周实验组与对照组最大载荷平均分别为（48．76±6．03）和（56．17±4．10）N、（65．64±4．49）和（74．67±7．69）N、（101．04±3．35）和（90．77±6．91）N、（131．35±6．91）和（150．61±21．41）N，两组比较差异均无统计学意义（P〉0．05）。结论两种细胞构建的组织工程化骨都可以较快地修复长骨临界大小节段性骨缺损，PMSCs与BMSCs具有相似的生物学特性和成骨特性，可作为骨组织工程的另一成体干细胞来源。Objective To compare osteogenic capacities of allogeneic bone marrow stromal cells （BMSCs） and planceta marrow stromal cells （PMSCs） to repair critical-sized segmental bone defects in rabbits. Methods Segmental osteoperiosteal defects of 1.5 cm were created surgically at bilateral radii in 24 rabbits. The defects at the left side were bridged with tissue engineered bone constructed by PMSCs （experimental group） while those at the right side were bridged with tissue engineered bone constructed by BMSCs （control group） . The rabbits were observed generally after the operation. At 2, 4, 8, 12 weeks postoperatively, X-rays of the bilateral radii were taken for radiographic assessments and the animals were sacrificed to harvest the involved radii. After macroscopic examination, the defects and their adjacent bone were prepared for histological and scanning electron microscopy observations as well as for biomechanical tests. Results The radiographic scores at 2, 4, 8, 12 weeks postoperatively for the experimental and control groups were respectively 3.72 ± 0.24 versus 3.49 ± 0.29, 6.24 ± 0. 41 versus 5.91 ± 0.45, 8.15 ± 0.67 versus 8.87±0.59, and 11.39±0.86 versus 12.04±0.93, with no significant differences （P〉 0.05）. Both intramembranous and endochondral ossifications were involved in the osteogenic processes of the bone defect repair, but the latter dominated the process. The maximum loads at 2, 4, 8, 12 weeks postoperatively for the experimental and control groups were respectively 48.76 -± 6.03 N versus 56. 17 ± 4.10 N, 65.64 ± 4.49 N versus 74.67 ± 7.69 N, 101.04 ± 3.35 N versus 90.77 ± 6.91 N, and 131.35 ± 6.91 N versus 150.61 ± 21.41 N, with no significant differences（ P 〉 0. 05）. Conclusions Tissue engineered bone constructed with either allogeneic PMSCs or BMSCs is capable of healing critical-sized segmental bone defects in rabbits. Since PMSCs have similar biological characteristics and osteogenic capacity compared with BMSCs, they can be used as a new source of see

关 键 词：间质干细胞 胎盘 骨髓 组织工程 兔 

分 类 号：R6[医药卫生—外科学]