一种新的造血干细胞获取方法的建立  

Development of a Novel Method for Harvest of Hematopoietic Stem Cells

作　　者：武晓云[1] 王世立[1] 

机构地区：[1]山东省医药生物技术研究中心国家卫生部生物技术药物重点实验室山东省现代医用药物与技术重点实验室,济南250062

出　　处：《中国生物制品学杂志》2011年第11期1344-1347,共4页Chinese Journal of Biologicals

基　　金：国家自然科学基金项目(30940065);山东省自然基金(Y2008C06)

摘　　要：目的建立一种新的造血干细胞获取方法,为临床应用提供实验依据。方法将明胶海绵移植到小鼠后肢的大腿内侧肌间隙,12 d后分离明胶海绵中的迁移细胞。采用流式细胞术和免疫荧光法检测迁移细胞和骨髓细胞中CD34+、Sca-1+和CD34+Sca-1+细胞的表达,利用体外造血克隆分析迁移细胞和骨髓细胞中造血克隆的密度。结果迁移细胞的数量在移植12 d时达最大;迁移细胞中CD34+、Sca-1+和CD34+Sca-1+细胞的比例明显高于骨髓细胞(P<0.05),造血克隆比例也明显高于骨髓(P<0.01)。结论利用生物材料明胶海绵成功建立了一种高效的造血干细胞获取方法,可实现自体细胞治疗。Objective To develop a novel method for harvest of hematopoietic stem cells and provide an experimental basis for clinical application. Methods Gelatin sponges （GS） were implanted into the spatium intermusculare of mice hind limbs, in Which the migrating cells were isolated 12 d later. The expressions of CD34, Sca-1, and CD34Sca-1 cells in migrating cells （MCs） and bone marrow cells （BMCs） were determined by flow eytometry and IFA, and the density of hematopoietic colonies by colony-forming cell assay in vitro. Results The count of migrating cells reached the maximum 12 d after transplantation. The proportions of CD34＋, Sca-I and CD34＊Sca-1 cells （each P 〈 0. 05） as well as the density of hematopoietic colonies were significantly higher in MCs than in BMCs （P 〈 0. 01 ）. Conclusion An effective method for harvest of hematopoietic stem cells was successfully developed with GS as a biomaterial, by which autologous cell therapy might be feasible.

关 键 词：明胶海绵 造血干细胞 抗原 CD34 造血克隆 

分 类 号：R457.7[医药卫生—治疗学]