梯形微板法在ABO反定型中的应用  

Application of Trapezoidal Microplate Method to Reverse ABO Grouping

作　　者：杜雪宁 刘芳兰 王振卿 张来玉 

机构地区：[1]衡水市中心血站,河北衡水053000

出　　处：《中国生物制品学杂志》2011年第11期1366-1367,1376,共3页Chinese Journal of Biologicals

摘　　要：目的评价梯形微板法在ABO反定型中的应用。方法采用梯形微板进行血型抗原抗体免疫学反应,利用高科技摄像数码分析技术进行凝集判别,确定血型,分析该方法的准确性、可靠性、重复性和脂血抗干扰性,同时以U型微板法作平行对照。结果梯形微板法的准确性为99.97%,可靠性较高,重复性为100%,且能有效避免脂血的干扰,各项指标均优于U型微板法。结论梯形微板法在ABO反定型鉴定中是一项准确性高、稳定性好的检测手段,更适合于血站系统的大批量标本的结果处理。Objective To evaluate the application of trapezoidal microplate method to reverse ABO grouping. Methods Immunological reaction of blood group antigen and antibody were carried out on trapezoidal mieroplate, and the blood group was determined by judging the agglutination by using high-tech camera digital analysis. The method was evaluated for accuracy, reliability, reproducibility and resistance to interference of blood lipid, using U-shaped mieroplate method as control. Results The accuracy and reproducibility of trapezoidal microplate method were 99. 97% and 100% respectively, and the interference of blood lipid was effectively avoided. All the indexed of the method were superior to those of U-shaped mieroplate method. Conclusion Trapezoidal mieroplate method is a high accurate and stable method for reverse ABO grouping, which is more suitable for the treatment of a large quantity of samples in blood station system.

关 键 词：ABO正反定型 梯形微板法 U型微板法 

分 类 号：R457.11[医药卫生—治疗学]