SP-D基因多态性和蛋白水平与成年女性尿路感染易感性的相关性研究  

Association of Gene Polymorphism and Protein Level of Surfactant Protein D with Urinary Tract Infection Susceptibility in Adult Women

作　　者：刘娇[1] 梁伟[2] 丁国华[2] 王桂荣[3] 

机构地区：[1]武汉大学人民医院重症医学科,湖北武汉430060 [2]武汉大学人民医院肾内科,湖北武汉430060 [3]美国宾夕法尼亚州立大学医学院细胞和分子生物学系,美国费城17033

出　　处：《武汉大学学报（医学版）》2011年第6期769-772,782,共5页Medical Journal of Wuhan University

基　　金：国家自然科学基金资助项目(编号:81070556)

摘　　要：目的:研究表面活性蛋白-D(SP-D)基因多态性和蛋白水平与汉族成年女性尿路感染(UTI)易感性的相关性。方法:应用序列特异性引物聚合酶链反应(PCR-SSP)的方法对32例女性尿路感染易感患者组和30例女性健康对照者组进行SP-D-Thr11Met、SP-D-Thr160Ala外显子位点的基因多态性检测;同时应用ELISA方法分析尿路感染易感患者和健康对照者血液中SP-D蛋白的水平。结果:SP-D-Thr11Met位点尿路感染女性易感患者Thr/Thr,Thr/Met,Met/Met基因型频率分别为28.1%,56.3%,15.6%,而健康对照组分别为36.7%,50.0%,13.3%;尿路感染易感组11Thr和11Met等位基因的频率分别为43.8%,56.2%,健康对照组的频率分别为38.3%,61.7%,两组间差异无统计学意义(P>0.05)。SP-D-Thr160Ala位点尿路感染女性易感患者Thr/Thr,Thr/Ala,Ala/Ala基因型频率分别为18.8%,34.4%和46.9%,而健康对照组分别为10.0%,33.3%和56.7%;尿路感染易感组160Thr和160Ala等位基因的频率分别为35.5%,64.4%,健康对照组的频率分别为26.7%,73.3%,两组间差异无统计学意义(P>0.05)。尿路感染易感患者血中SP-D蛋白浓度明显高于健康对照组血中SP-D蛋白浓度(P<0.05)。结论:SP-D-Thr11Met和SP-D-Thr160Ala位点等位基因的变异可能不能导致汉族成年女性对尿路感染易感;而血中SP-D蛋白的水平可能与成年女性尿路感染易感性相关。Objective： To evaluate the association of surfactant protein D（SP-D） gene polymorphisms and its protein levels with the susceptible female patients of urinary tract infection（UTI） in Han population.Methods： Polymorphisms of SP-D-Thr11Met and SP-D-Thr160Ala loci were analyzed by sequence special primer polymerase chain reaction（PCR-SSP）.Serum SP-D protein levels were measured using ELISA method.Results： The frequencies of Thr/Thr,Thr/Met,and Met/Met genotypes at SP-D-Thr11Met locus were 28.1%,56.3% and 15.6% respectively in UTI susceptible patients,and 36.7%,50.0% and 13.3% respectively in health control group.Allelic frequencies of 11Thr and 11Met at this SNP site were 43.8% and 56.2% respectively in UTI susceptible patients,as well as 38.3% and 61.7% respectively in control group.The frequencies of Thr/Thr,Thr/Ala and Ala/Ala genotypes at SP-D-Thr160Ala locus were 18.8%,34.4%,and 46.9% respectively in UTI susceptible patients,while in control group they were 10.0%,33.3% and 56.7%,respectively.Allelic frequencies of 160Thr and 160Ala at this SNP site were 35.5% and 64.4% respectively in UTI susceptible patients,as well as 26.7% and 73.3% respectively in control group.The frequencies of genotypes and alleles at SP-D-Thr11Met and SP-D-Thr160Ala loci showed no significant difference between the two groups（P0.05）.The serum SP-D levels were significantly increased in UTI patients as compared with those in controls（P0.05）.Conclusion： SP-D-Thr11Met and SP-D-Thr160Ala loci gene mutations are not risk factors for r-UTI in Chinese women,however the blood SP-D level was associated with UTI susceptibility in Chinese adult women.

关 键 词：尿路感染 表面活性蛋白-D 基因多态性 蛋白水平 

分 类 号：R691.3[医药卫生—泌尿科学]